The uptake and transport behavior of berberine in Coptidis Rhizoma extract through rat primary cultured cortical neurons
The uptake and transport behavior of berberine in Coptidis Rhizoma extract through rat primary cultured cortical neurons
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DOI：
10.1016/j.neulet.2004.12.050
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发表时间：
2005-05-06
影响因子：
2.5
通讯作者：
Du, LJ
中科院分区：
文献类型：
--
作者：
Wang, XL;Xing, DM;Du, LJ
本研究旨在探讨黄连提取物中小檗碱是否能在原代培养的大脑神经元中转运，并研究小檗碱在神经元中的摄取和转运行为。结果表明，在0.3822 ~ 38.22 μ g/mL范围内，随着黄连提取物中小檗碱浓度的增加，神经元对小檗碱的摄取呈线性增加。黄连提取物的培养液对神经元的输送具有浓度依赖性和时间依赖性。培养基中小檗碱的浓度与细胞中小檗碱的浓度呈正相关，这意味着细胞对小檗碱的吸收随着提取物剂量的增加而增加。但培养基中小檗碱浓度与渗透比呈负相关趋势。提示黄连提取物中的小檗碱以可变速率进入神经元。2004爱思唯尔爱尔兰有限公司版权所有。
The objective of this study was to investigate whether berberine in Coptidis Rhizoma extract could be transported in primary cultured cerebral neurons and to study the uptake and transport behavior of berberine through the neurons. The present results showed that the uptake of berberine by the neurons increased linearly with increasing concentration of berberine in Coptidis Rhizoma extract over the range of 0.3822-38.22 mu g/mL. Berberine could be transported into the neurons in a concentration-dependent and time-dependent manner when the neurons were fed with the incubation medium containing Coptidis Rhizonra extract. There was a positive correlation between the concentration of berberine in the medium and in the cell, which means that uptake of berberine increased in the cell along with higher dose of the extract. But there was a negative correlation tendency between the concentration of berberine in the medium and the penetration ratio. It is suggested that berberine in Coptidis Rhizoma extract was transported into the neurons at a variable rate. (c) 2004 Elsevier Ireland Ltd. All rights reserved.