Limited influence of hospital wastewater on the microbiome and resistome of wastewater in a community sewerage system
Limited influence of hospital wastewater on the microbiome and resistome of wastewater in a community sewerage system
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DOI：
10.1093/femsec/fiy087
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发表时间：
2018-07-01
影响因子：
4.2
通讯作者：
van Schaik, Willem
中科院分区：
文献类型：
--
作者：
Buelow, Elena;Bayjanov, Jumamurat R.;van Schaik, Willem
污水处理厂的出水是环境中耐药菌和耐药基因的点源。医院污水可能会导致ARB和ARG的传播，因为它含有住院患者的粪便和尿液，这些患者比一般人群更容易感染多重耐药细菌。然而，是否医院污水显着贡献的数量和多样性的ARGs在一般的污水系统尚未确定，在这里，我们采用了培养的独立技术，即16S rRNA基因测序和纳升规模的定量PCR，以评估医院污水作为点源的ARGs在污水系统中的作用，通过比较医院污水中的微生物组成和ARGs水平，污水处理厂进水中有和没有医院污水。与其他地点相比，医院污水中人类相关细菌最丰富，ARGs的相对水平最高。然而，ARGs的相对丰度在有和没有医院污水的污水处理厂的进水中是相当的，这表明医院对调查的污水处理系统中ARGs的数量和多样性没有重要贡献。
Effluents from wastewater treatment plants (WWTPs) have been proposed to act as point sources of antibiotic-resistant bacteria (ARB) and antimicrobial resistance genes (ARGs) in the environment. Hospital sewage may contribute to the spread of ARB and ARGs as it contains the feces and urine of hospitalized patients, who are more frequently colonized with multi-drug resistant bacteria than the general population. However, whether hospital sewage noticeably contributes to the quantity and diversity of ARGs in the general sewerage system has not yet been determined.Here, we employed culture-independent techniques, namely 16S rRNA gene sequencing and nanolitre-scale quantitative PCRs, to assess the role of hospital effluent as a point source of ARGs in the sewerage system, through comparing microbiota composition and levels of ARGs in hospital sewage with WWTP influent with and without hospital sewage. Compared to other sites, hospital sewage was richest in human-associated bacteria and contained the highest relative levels of ARGs. Yet, the relative abundance of ARGs was comparable in the influent of WWTPs with and without hospital sewage, suggesting that hospitals do not contribute importantly to the quantity and diversity of ARGs in the investigated sewerage system.