Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity
Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity
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DOI：
10.1038/labinvest.2015.148
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发表时间：
2016-03-01
影响因子：
5
通讯作者：
Lassmann, Silke
中科院分区：
文献类型：
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作者：
Ahrens, Theresa D.;Timme, Sylvia;Lassmann, Silke
我们之前表明组蛋白脱乙酰酶抑制剂（HDACi）和5-氮杂胞苷（AZA）治疗选择性诱导食管癌细胞的细胞死亡。然而，癌症选择性的机制仍不清楚。在这里，我们检查了 HDACi/AZA 治疗的癌症选择性是否是由食管癌细胞中的硫氧还蛋白 (Trx) 系统和活性氧 (ROS) 介导的。为此，我们首先通过免疫组织化学分析了 37 名食管癌患者的人体组织标本中的 Trx、Trx 相互作用蛋白 (TXNIP) 和 Trx 还原酶 (TXNRD)。这揭示了与正常上皮细胞相比，食管癌细胞中核 Trx 的丢失或至少减少（P
We previously showed that histone deacetylase inhibitor (HDACi) and 5-azacytidine (AZA) treatment selectively induced cell death of esophageal cancer cells. The mechanisms of cancer selectivity, however, remained unclear. Here we examined whether the cancer selectivity of HDACi/AZA treatment is mediated by the thioredoxin (Trx) system and reactive oxygen species (ROS) in esophageal cancer cells. For this, we first analyzed human tissue specimens of 37 esophageal cancer patients by immunohistochemistry for Trx, Trx-interacting protein (TXNIP) and Trx reductase (TXNRD). This revealed a loss or at least reduction of nuclear Trx in esophageal cancer cells, compared with normal epithelial cells (P