2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS IN THE FIRST DIMENSION (IPG-DALT) - THE STATE-OF-THE-ART AND THE CONTROVERSY OF VERTICAL VERSUS HORIZONTAL SYSTEMS
2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS IN THE FIRST DIMENSION (IPG-DALT) - THE STATE-OF-THE-ART AND THE CONTROVERSY OF VERTICAL VERSUS HORIZONTAL SYSTEMS
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DOI：
10.1002/elps.11501601183
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发表时间：
1995-07-01
期刊：
影响因子：
2.9
通讯作者：
WEISS, W
中科院分区：
文献类型：
--
作者：
GORG, A;BOGUTH, G;WEISS, W
在1988年（A. Gorg et al., electrophoresis 1988, 9,531 -546）建立了一维固定化pH梯度二维电泳（IPG-Dalt）的基本方案后，对实际IPG-Dalt方案的一些关键参数以及用水平和垂直二维十二烷基硫酸钠电泳得到的结果进行了论证和讨论。
After having established the basic protocol of two-dimensional electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt) in 1988 (A. Gorg et al., Electrophoresis 1988, 9, 531-546), some critical parameters of the actual IPG-Dalt protocols as well as the results obtained with horizontal and vertical second-dimensional sodium dodecyl sulfate-electrophoresis are demonstrated and discussed.