Transcriptome analysis of an arsenite-/antimonite-oxidizer, Bosea sp. AS-1 reveals the importance of the type 4 secretion system in antimony resistance
Transcriptome analysis of an arsenite-/antimonite-oxidizer, Bosea sp. AS-1 reveals the importance of the type 4 secretion system in antimony resistance
复制标题
亚砷酸盐/锑酸盐氧化剂 Bosea sp 的转录组分析。
DOI：
10.1016/j.scitotenv.2022.154168
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发表时间：
2022-03-04
影响因子：
9.8
通讯作者：
Lu，Xiaolu
中科院分区：
文献类型：
--
作者：
Wu，Yanmei;Xiang，Li;Lu，Xiaolu
Boseasp。As -1是本课题组先前从锡矿山锑矿区分离到的一种亚砷矿[As(III)]和锑矿[Sb(III)]氧化剂。我们之前的研究表明，boseasp。As -1对As（III）或Sb（III）具有不同碳源氧化的偏好，提示细菌在As(III)-或Sb（III）污染环境中生存可能利用了不同的代谢机制。在这里，我们进行了全基因组和转录组测序，以揭示boseasp利用的分子机制。As -1抗As（III）或Sb（III）。我们发现As- 1在其基因组中获得了多种As-和Sb抗性基因，并可能通过调节As和Sb代谢、细菌分泌系统、氧化磷酸化、TCA循环和细菌鞭毛运动等多种途径来抵抗As（III）或Sb（III）。有趣的是，我们发现IV型分泌系统（T4SS）的基因在Sb（III）的作用下被激活，在AS-1中抑制T4SS活性会显著降低其氧化效率和对Sb（III）的耐受性。据我们所知，这是第一个显示Sb激活T4SS基因并直接参与细菌Sb抗性的研究。我们的研究结果表明，T4SS是细菌中一个重要的锑抗性因子，可能有助于我们了解锑抗性基因在环境中的传播。
Boseasp. AS-1 is an arsenite [As(III)] and antimonite [Sb(III)] oxidizer previously isolated by our group from the Xikuangshan Antimony (Sb) Mine area. Our previous study showed thatBoseasp. AS-1 had a preference for oxidizing As(III) or Sb(III) with different carbon sources, which suggested that different metabolic mechanisms may be utilized by the bacteria to survive in As(III)- or Sb(III)-contaminated environments. Here, we conducted whole-genome and transcriptome sequencing to reveal the molecular mechanisms utilized byBoseasp. AS-1 to resist As(III) or Sb(III). We discovered that AS-1 acquired various As- and Sb-resistant genes in its genome and might resist As(III) or Sb(III) through the regulation of multiple pathways, such as As and Sb metabolism, the bacterial secretion system, oxidative phosphorylation, the TCA cycle and bacterial flagellar motility. Interestingly, we discovered that genes of the type IV secretion system (T4SS) were activated in response to Sb(III), and inhibiting T4SS activity in AS-1 dramatically reduced its oxidation efficiency and tolerance to Sb(III). To our knowledge, this is the first study showing the activation of T4SS genes by Sb and a direct involvement of T4SS in bacterial Sb resistance. Our findings establish the T4SS as an important Sb resistance factor in bacteria and may help us understand the spread of Sb resistance genes in the environment.