Efficient formation of giant liposomes through the gentle hydration of phosphatidylcholine films doped with sugar
Efficient formation of giant liposomes through the gentle hydration of phosphatidylcholine films doped with sugar
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DOI：
10.1016/j.colsurfb.2008.09.023
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发表时间：
2009-01-01
影响因子：
5.8
通讯作者：
Yoshimura, Tetsuro
中科院分区：
文献类型：
--
作者：
Tsumoto, Kanta;Matsuo, Hideki;Yoshimura, Tetsuro
巨脂质体或巨囊泡是由磷脂双层包围的细胞大小（类似于5-100 μ m）的隔室，经常用于生物学研究。它们通常使用水合方法，“电铸”和“温和水合（或自然溶胀）"产生，其中磷脂的干燥层状膜与水溶液水合。然而，在温和的水合作用下，很难从静电中性磷脂制备巨大的脂质体，因为脂质体不能相互排斥。在这项研究中，我们证明了使用中性磷脂（二油酰磷脂酰胆碱）的温和水化有效地形成巨大的脂质体。DOPC）干膜，其掺杂有非电解质单糖（葡萄糖、甘露糖和果糖）。将DOPC和这种糖在有机溶剂（氯仿/甲醇）中的混合物蒸发以形成膜，然后将其用蒸馏水或含有氯化钠的Tris缓冲液水合。在这些条件下，巨大的脂质体自发地迅速形成，并假设一个肿胀的细胞大小的球形形状与低层状，而从纯DOPC膜的巨大的脂质体具有多层脂质层，杂项形状和较小的尺寸。这一观察结果表明，DOPC的巨型单层囊泡（GUV）可以有效地通过温和的水合含糖脂质干膜，因为脂质层之间的排斥作用增强溶解的糖诱导的渗透。(C)2008 Elsevier B. V.保留所有权利。
Giant liposomes, or giant vesicles, are cell-size (similar to 5-100 mu m) compartments enclosed with phospholipid bilayers, and have often been used in biological research. They are usually generated using hydration methods, "electroformation" and "gentle hydration (or natural swelling)", in which dry lamellar films of phospholipids are hydrated with aqueous solutions. In gentle hydration, however, giant liposomes are difficult to prepare from an electrostatically neutral phospholipid because lipid lamellae cannot repel each other. In this study, we demonstrate the efficient formation of giant liposomes using the gentle hydration of neutral phospholipid (dioleoyl phosphatidylcholine. DOPC) dry films doped with nonelectrolytic monosaccharides (glucose, mannose, and fructose). A mixture of DOPC and such a sugar in an organic solvent (chloroform/methanol) was evaporated to form the films, which were then hydrated with distilled water or Tris buffers containing sodium chloride. Under these conditions, giant liposomes spontaneously formed rapidly and assumed a swollen cell-sized spherical shape with low lamellarity, whereas giant liposomes from pure DOPC films had multilamellar lipid layers, miscellaneous shapes and smaller sizes. This observation indicates that giant unilamellar vesicles (GUVs) of DOPC can be obtained efficiently through the gentle hydration of sugar-containing lipid dry films because repulsion between lipid lamellae is enhanced by the osmosis induced by dissolved sugar. (C) 2008 Elsevier B.V. All rights reserved.