Molecular Adaptations to Aerobic Exercise Training in Skeletal Muscle of Older Women
Molecular Adaptations to Aerobic Exercise Training in Skeletal Muscle of Older Women
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DOI：
10.1093/gerona/glq109
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发表时间：
2010-11-01
影响因子：
5.1
通讯作者：
Harber, Matthew P.
中科院分区：
文献类型：
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作者：
Konopka, Adam R.;Douglass, Matthew D.;Harber, Matthew P.
我们最近的研究表明，12周的渐进式有氧运动训练可以改善老年女性的整体肌肉大小和功能。对9名老年女性(70+/-2岁)在有氧训练前和有氧训练12周后，在自行车测功机上采集肌肉活组织标本，以测定肌原、蛋白水解物和线粒体因子的基础信使RNA水平和蛋白质含量。有氧训练使有氧能力增加30+/-9%，全肌横截面积增加11+/-2%，全肌力产生增加29+/-8%。有氧训练后FOXO3a、myostatin、HSP70和MRF4的基础信使RNA水平降低(p<；0.05)。训练后FOXO3a、FOXO3a磷酸化和HSP70蛋白含量无明显变化。线粒体COX IV蛋白在有氧训练后升高33+/-7%，而PGC-1α蛋白含量降低20+/-5%(P<；0.05)。这些数据表明FOXO3a和肌肉生长抑素信使RNA的减少可能与运动诱导的肌肉肥大有关。此外，似乎线粒体的生物发生可以通过有氧训练发生在老年女性身上，而不是依赖于PGC-1α蛋白的增加。有氧运动训练改变了与骨骼肌调节有关的分子因素，这支持了有氧训练对改善老年女性肌肉健康的有益作用。
We have recently shown that 12 weeks of progressive aerobic exercise training improves whole-muscle size and function in older women. The purpose of this investigation was to evaluate molecular markers that may be associated with muscle hypertrophy after aerobic training in aging skeletal muscle.Muscle biopsies were obtained before and after 12 weeks of aerobic exercise training on a cycle ergometer in nine older women (70 +/- 2 years) to determine basal levels of messenger RNA and protein content of select myogenic, proteolytic, and mitochondrial factors.The training program increased (p < .05) aerobic capacity 30 +/- 9%, whole-muscle cross-sectional area 11 +/- 2%, and whole-muscle force production 29 +/- 8%. Basal messenger RNA levels of FOXO3A, myostatin, HSP70, and MRF4 were lower (p < .05) after aerobic training. FOXO3A, FOXO3A phosphorylation, and HSP70 protein content were unaltered after training. Mitochondrial protein COX IV was elevated (p < .05) 33 +/- 7% after aerobic training, whereas PGC-1 alpha protein content was 20 +/- 5% lower (p < .05).These data suggest that reductions in FOXO3A and myostatin messenger RNA are potentially associated with exercise-induced muscle hypertrophy. Additionally, it appears that mitochondrial biogenesis can occur with aerobic training in older women independent of increased PGC-1 alpha protein. Aerobic exercise training alters molecular factors related to the regulation of skeletal muscle, which supports the beneficial role of aerobic training for improving muscle health in older women.