Anterior Cruciate Ligament Reconstruction Using Chitin-coated Fabrics in a Rabbit Model
Anterior Cruciate Ligament Reconstruction Using Chitin-coated Fabrics in a Rabbit Model
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DOI：
10.1111/j.1525-1594.2009.00786.x
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Takakuda, Kazuo
中科院分区：
文献类型：
--
作者：
Kawai, Tomoyuki;Yamada, Takeki;Takakuda, Kazuo
在兔模型中进行实验性前交叉韧带（ACL）重建，其中使用几丁质涂层聚酯移植物作为支架，并使用未涂层移植物作为对照移植物。植入8周后，进行膝关节的力学测试和移植物和周围组织的组织计量学测量。股骨-移植物-胫骨样本的拉伸试验表明，用涂层移植物处理的膝关节具有42.2 +/- 12.7 N的峰值阻力，显著大于用对照移植物处理的膝关节的19.2 +/- 15.3 N。组织学测量显示，股骨隧道中涂层移植物截面内的骨组织面积为3.43 +/- 1.73 mm（2），显著大于对照移植物的0.29 +/- 0.37 mm（2）。同样，位于关节腔内的涂层移植物中间物质内和周围的软组织面积显著大于对照移植物。甲壳素涂层促进了股骨隧道内骨组织和关节腔内软组织的形成，并增加了移植物与骨的附着强度。因此，证明了甲壳质涂层用于ACL重建支架的功效。
Experimental anterior cruciate ligament (ACL) reconstruction was carried out in a rabbit model, in which a chitin-coated polyester graft was used as the scaffold, and a noncoated graft was used as a control graft. After 8 weeks implantation, a mechanical test of the knee and histometric measurement of the graft and surrounding tissues were carried out. A tensile test of the femur-graft-tibia specimen showed that the knee treated with the coated graft had a peak resistance force of 42.2 +/- 12.7 N, which was significantly greater than the 19.2 +/- 15.3 N of the knee treated with the control graft. The histometric measurement revealed that the area of bone tissue within the section of the coated graft in the femoral bone tunnel was 3.43 +/- 1.73 mm(2), which was significantly greater than the area of 0.29 +/- 0.37 mm(2) of the control graft. Similarly, the area of soft tissue within and around the midsubstance of the coated graft located in the articular cavity was significantly greater than that of the control graft. The chitin coating enhanced the formation of bone tissue in the femoral bone tunnel and soft tissue in the articular cavity, and increased the attachment strength of the graft to the bone. Thus, the efficacy of the chitin coating for the ACL reconstruction scaffolds was demonstrated.