A T-to-G transversion at nucleotide-567 upstream of HBG2 in a GATA-1 binding motif is associated with elevated hemoglobin F
A T-to-G transversion at nucleotide-567 upstream of HBG2 in a GATA-1 binding motif is associated with elevated hemoglobin F
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DOI：
10.1128/mcb.00071-08
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发表时间：
2008-07-01
影响因子：
5.3
通讯作者：
Chui, David H. K.
中科院分区：
文献类型：
--
作者：
Chen, Zhiyi;Luo, Hong-Yuan;Chui, David H. K.
Increased fetal hemoglobin (Hb F; alpha(2)gamma(2)) production in adults can ameliorate the clinical severity of sickle cell disease and beta-thalassemia major. Thus, understanding the regulation of gamma-globin gene expression and its silencing in adults has potential therapeutic implications. We studied a father and son in an Iranian-American family who had elevated Hb F levels and found a novel T-to-G transversion at nucleotide (nt) -567 of the HBG2 promoter. This mutation alters a GATA-1 binding motif to a GAGA sequence located within a previously identified silencing element. DNA-protein binding assays showed that the GATA motif of interest is capable of binding GATA-1 transcription factor in vitro and in vivo. Truncation analyses of the HBG2 promoter linked to a luciferase reporter gene revealed a negative regulatory activity present between nt -675 and -526. In addition, the T-to-G mutation at the GATA motif increased the promoter activity by two- to threefold in transiently transfected erythroid cell lines. The binding motif is uniquely conserved in simian primates with a fetal pattern of gamma-globin gene expression. These results suggest that the GATA motif under study has a functional role in silencing gamma-globin gene expression in adults. The T-to-G mutation in this motif disrupts GATA-1 binding and the associated repressor complex, abolishing its silencing effect and resulting in the up-regulation of gamma-globin gene expression in adults.
