Contrast-enhanced CT imaging as a non-destructive tool for ex vivo examination of the biochemical content and structure of the human meniscus.
Contrast-enhanced CT imaging as a non-destructive tool for ex vivo examination of the biochemical content and structure of the human meniscus.
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对比增强 CT 成像作为一种非破坏性工具，用于离体检查人类半月板的生化内容和结构。
DOI：
10.1002/jor.23337
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Snyder,BrianD
中科院分区：
文献类型：
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作者：
Oh,DanielJ;Lakin,BenjaminA;Stewart,RachelC;Wiewiorski,Martin;Freedman,JonathanD;Grinstaff,MarkW;Snyder,BrianD
用于研究半月板内容和结构的生化和组织病理学技术具有破坏性且耗时。因此，本研究评估了使用碘化造影剂 CA4+ 和碘沙明的对比增强计算机断层扫描 (CECT) 衰减和造影剂通量是否与人类半月板中的糖胺聚糖 (GAG) 含量/分布和水含量相关。通过比较 CECT 彩色图与 Safranin-O 染色的组织学切片，定性确定绘制半月板 GAG 分布图的最佳 ioxaglate 和 CA4+ 造影剂浓度。使用两种造影剂确定不同时间点的 CECT 衰减和 GAG 含量、CECT 衰减和含水量以及通量和含水量之间的关联。还对每个原生表面进行了深度分析，以检查造影剂扩散动力学和平衡分配的差异。 Ioxaglate 和 CA4+ 的 GAG 描述的最佳浓度分别为 ≥80 和 12mgI/ml。使用这些浓度，在所有扩散时间点（1-48 h），ioxaglate 衰减和 GAG 含量之间都发现弱到中度的关联，而早在 7 h 时，CA4+ 衰减和 GAG 含量之间就观察到强而显着的关联（R2≥ 0.67），在平衡时间点（48 h，R2= 0.81）最强。两种药剂的 CECT 衰减与含水量没有显着相关，但 CA4+ 通量与含水量相关 (R2= 0.56–0.64)。与传统的生化和组织病理学方法相比，CECT 是一种有前景的无损成像技术，可用于离体评估半月板 GAG 浓度和水含量。 © 2017 骨科研究会。由 Wiley periodicals, Inc. 出版。J Orthop Res 35:1018–1028，2017 年。
The biochemical and histopathological techniques used to investigate meniscal content and structure are destructive and time‐consuming. Therefore, this study evaluated whether contrast‐enhanced computed tomography (CECT) attenuation and contrast agent flux using the iodinated contrast agents CA4+ and ioxaglate correlate with the glycosaminoglycan (GAG) content/distribution and water content in human menisci. The optimal ioxaglate and CA4+ contrast agent concentrations for mapping meniscal GAG distribution were qualitatively determined by comparison of CECT color maps with Safranin‐O stained histological sections. The associations between CECT attenuation and GAG content, CECT attenuation and water content, and flux and water content at various time points were determined using both contrast agents. Depth‐wise analyses were also performed through each of the native surfaces to examine differences in contrast agent diffusion kinetics and equilibrium partitioning. The optimal concentrations for GAG depiction for ioxaglate and CA4+ were ≥80 and 12 mgI/ml, respectively. Using these concentrations, weak to moderate associations were found between ioxaglate attenuation and GAG content at all diffusion time points (1–48 h), while strong and significant associations were observed between CA4+ attenuation and GAG content as early as 7 h (R2≥ 0.67), being strongest at the equilibrium time point (48 h,R2= 0.81). CECT attenuation for both agents did not significantly correlate with water content, but CA4+ flux correlated with water content (R2= 0.56–0.64). CECT is a promising, non‐destructive imaging technique for ex vivo assessment of meniscal GAG concentration and water content compared to traditional biochemical and histopathological methods. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1018–1028, 2017.