β-Aminobutyric Acid Priming Acquisition and Defense Response of Mango Fruit to Colletotrichum gloeosporioides Infection Based on Quantitative Proteomics
β-Aminobutyric Acid Priming Acquisition and Defense Response of Mango Fruit to Colletotrichum gloeosporioides Infection Based on Quantitative Proteomics
复制标题
基于定量蛋白质组学的芒果果实对胶孢炭疽菌感染的β-氨基丁酸启动获取和防御反应
DOI：
10.3390/cells8091029
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发表时间：
2019-09-01
期刊：
影响因子：
6
通讯作者：
Jiang, Yueming
中科院分区：
文献类型：
--
作者：
Li, Taotao;Fan, Panhui;Jiang, Yueming
β-氨基丁酸（BABA）是一种新型的环境友好型植物抗病诱导剂。然而，BABA诱导的启动防御的分子机制尚未完全了解。本文以芒果-炭疽菌互作系统为研究对象，采用基于iTRAQ的蛋白质组学方法，对ABA诱导的芒果抗病性启动机制进行了全面分析。结果表明，BABA处理能有效地抑制炭疽病菌的扩展。芒果果实中的类粘孢子菌。蛋白质组学结果表明，在ABA引发的芒果果实中，C。此外，我们还证实了非特异性脂转移蛋白（nsLTP）参与了引发初期的启动获得和记忆。同时，在引发的芒果果实中发现了“春天效应”，表现为引发期防御相关蛋白的抑制，但在病原菌的作用下，防御反应的激活程度比未引发的果实更强。作为一种节能策略，BABA诱导的引发也可能改变糖代谢，为次生代谢产物的生物合成提供更多的骨架。本研究为阐明ABA诱导采后果实引发防御机制提供了新的线索。
beta-aminobutyric acid (BABA) is a new environmentally friendly agent to induce disease resistance by priming of defense in plants. However, molecular mechanisms underlying BABA-induced priming defense are not fully understood. Here, comprehensive analysis of priming mechanism of BABA-induced resistance was investigated based on mango-Colletotrichum gloeosporioides interaction system using iTRAQ-based proteome approach. Results showed that BABA treatments effectively inhibited the expansion of anthracnose caused by C. gleosporioides in mango fruit. Proteomic results revealed that stronger response to pathogen in BABA-primed mango fruit after C. gleosporioides inoculation might be attributed to differentially accumulated proteins involved in secondary metabolism, defense signaling and response, transcriptional regulation, protein post-translational modification, etc. Additionally, we testified the involvement of non-specific lipid-transfer protein (nsLTP) in the priming acquisition at early priming stage and memory in BABA-primed mango fruit. Meanwhile, spring effect was found in the primed mango fruit, indicated by inhibition of defense-related proteins at priming phase but stronger activation of defense response when exposure to pathogen compared with non-primed fruit. As an energy-saving strategy, BABA-induced priming might also alter sugar metabolism to provide more backbone for secondary metabolites biosynthesis. In sum, this study provided new clues to elucidate the mechanism of BABA-induced priming defense in harvested fruit.