A study of natural IgG antibodies against ATP-binding cassette subfamily C member 3 in oral squamous cell carcinoma
A study of natural IgG antibodies against ATP-binding cassette subfamily C member 3 in oral squamous cell carcinoma
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口腔鳞状细胞癌中针对 ATP 结合盒亚家族 C 成员 3 的天然 IgG 抗体的研究
DOI：
10.4103/jcrt.jcrt_150_18
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Ying Hu
中科院分区：
文献类型：
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作者：
Xiu Liu;Zhicheng Huang;Ziji He;Qingyong Meng;Xiaoyu Wang;Ying Hu
目的：ATP结合盒亚家族C成员3（ABCC 3）参与多药耐药，并在某些实体瘤中过表达。最近的研究显示，肺癌和食管癌中循环的抗ABCC 3抗体增加。本体外研究旨在研究抗ABCC 3衍生肽抗原的天然IgG抗体对口腔鳞状细胞癌（OSCC）细胞增殖的影响，并促进OSCC患者有效治疗的发展。对象和方法：采用酶联免疫吸附试验检测人血浆中抗ABCC 3 IgG抗体。将两种OSCC细胞系CAL 27和SCC 15与抗ABCC 3 IgG阳性或阴性的20%血浆一起培养。CCK-8法检测细胞增殖，流式细胞仪检测细胞凋亡及细胞周期分布。通过逆转录酶定量实时聚合酶链反应分析ABCC 3基因在细胞系中的表达。结果如下：结果表明，血浆抗ABCC 3 IgG显著抑制CAL 27细胞的增殖，但不抑制SCC 15细胞，尽管ABCC 3在两种细胞系中表达。用抗ABCC 3 IgG阳性血浆处理的CAL 27细胞中凋亡细胞的比例显著高于用IgG阴性血浆处理的那些细胞。在用抗ABCC 3 IgG阳性血浆处理的CAL 27细胞中，细胞周期进程被阻滞。结论：我们的数据表明，人血浆抗ABCC 3 IgG可能是一种有前途的药物在抗口腔鳞癌治疗，虽然需要进一步的研究，以达到一个明确的结论。
Aims: ATP-binding cassette subfamily C member 3 (ABCC3) is involved in multidrug resistance and is overexpressed in some solid tumors. Recent work revealed an increase in circulating anti-ABCC3 antibodies in lung and esophageal cancers. This in vitro study was undertaken to investigate the effects of the natural IgG antibody against the ABCC3-derived peptide antigen on proliferation of oral squamous cell carcinoma (OSCC) cells and augment the development of efficient and effective treatments in patients with OSCC. Subjects and Methods: An in-house enzyme-linked immunosorbent assay was applied to detect anti-ABCC3 IgG antibody in human plasma. Two OSCC cell lines, CAL27 and SCC15, were cultured with 20% plasma either positive or negative for anti-ABCC3 IgG. Cell proliferation was quantified by the CCK-8 method, and cell apoptosis and cell cycle distribution were analyzed by flow cytometry. The expression of the ABCC3 gene in the cell lines was analyzed by reverse transcriptase quantitative real-time polymerase chain reaction. Results: The results showed that plasma anti-ABCC3 IgG significantly inhibited the proliferation of CAL27 cells but not SCC15 cells, although ABCC3 was expressed in both cell lines. The proportion of apoptotic cells was significantly higher in CAL27 cells treated with anti-ABCC3 IgG-positive plasma than in those treated with IgG-negative plasma. Cell cycle progression was arrested in CAL27 cells treated with anti-ABCC3 IgG-positive plasma. Conclusions: Our data suggest that human plasma anti-ABCC3 IgG may be a promising agent in anti-OSCC therapy, although further studies are needed to arrive at a definitive conclusion.