Ultra-small gadolinium oxide nanoparticles to image brain cancer cells in vivo with MRI
Ultra-small gadolinium oxide nanoparticles to image brain cancer cells in vivo with MRI
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DOI：
10.1002/cmmi.420
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发表时间：
2011-07-01
影响因子：
--
通讯作者：
Fortin, Marc-Andre
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文献类型：
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作者：
Faucher, Luc;Guay-Begin, Andree-Anne;Fortin, Marc-Andre
磁共振成像（MRI）中使用的大多数造影剂是基于稀土元素钆。基于钆的纳米颗粒可以在某些类型癌症的临床前诊断程序中找到有前途的应用，例如多形性胶质母细胞瘤。这是最恶性、最致命和最难获得的癌症形式之一。胶体氧化合成的最新进展导致了氧化钆的超小晶体（US-Gd 2 O3，2- 3 nm直径）的发展。到目前为止，这是所有含Gd纳米颗粒中最小和最小的。用足够量的这种化合物标记的癌细胞在T-1加权MRI图像中显得明亮。在这里，我们证明，US-Gd 2 O3可用于标记GL-261多形性胶质母细胞瘤细胞，然后使用MRI在体内进行定位和可视化。非常高量的Gd被有效地内化并保留在细胞中，如TEM和ICP-MS所证实的。使用鸡胚胎模型在1.5 T下在体内可视化标记的细胞。这是朝着MRI“正对比”细胞跟踪程序的发展又迈出了一步。版权所有（C）2010约翰威利父子有限公司
The majority of contrast agents used in magnetic resonance imaging (MRI) is based on the rare-earth element gadolinium. Gadolinium-based nanoparticles could find promising applications in pre-clinical diagnostic procedures of certain types of cancer, such as glioblastoma multiforme. This is one of the most malignant, lethal and poorly accessible forms of cancer. Recent advances in colloidal nanocrystal synthesis have led to the development of ultra-small crystals of gadolinium oxide (US-Gd2O3, 2-3nm diameter). As of today, this is the smallest and the densest of all Gd-containing nanoparticles. Cancer cells labeled with a sufficient quantity of this compound appear bright in T-1-weighted MRI images. Here we demonstrate that US-Gd2O3 can be used to label GL-261 glioblastoma multiforme cells, followed by localization and visualization in vivo using MRI. Very high amounts of Gd are efficiently internalized and retained in cells, as confirmed with TEM and ICP-MS. Labeled cells were visualized in vivo at 1.5 T using the chicken embryo model. This is one more step toward the development of "positively contrasted" cell tracking procedures with MRI. Copyright (C) 2010 John Wiley & Sons, Ltd.