Simultaneous Intramuscular And Intranasal Administration Of Chitosan Nanoparticles-Adjuvanted Chlamydia Vaccine Elicits Elevated Protective Responses In The Lung
Simultaneous Intramuscular And Intranasal Administration Of Chitosan Nanoparticles-Adjuvanted Chlamydia Vaccine Elicits Elevated Protective Responses In The Lung
复制标题
同时肌内和鼻内施用壳聚糖纳米颗粒佐剂衣原体疫苗可引起肺部保护反应增强
DOI：
10.2147/ijn.s218456
复制
发表时间：
2019-01-01
影响因子：
8
通讯作者：
Wu, Yimou
中科院分区：
文献类型：
--
作者：
Li, Yumeng;Wang, Chuan;Wu, Yimou
背景：鹦鹉热衣原体是一种与鹦鹉热/鸟类病密切相关的人畜共患细菌。接种疫苗已被公认为是抑制C.由于大多数人忽视了鹦鹉热的感染。方法：本研究设计了一种新的免疫策略，即同时（SIM）肌肉注射（IM）和鼻内注射（IN）衣原体疫苗。用壳聚糖纳米颗粒（CNP）作为佐剂的鹦鹉热抗原（Ag）。结果：Zeta电位为13.12mV、276.1nm的CNPs-Ags具有良好的稳定性和最佳的大小，其包封率为71.7%。SIM-CPN-Ag通过产生有意义的高水平IgG和分泌型伊加（sIgA）抗体介导更强的体液和粘膜应答。SIM途径还导致Ag特异性T细胞应答和脾细胞上清液中IFN-γ、IL-2、TNF-α和IL-17 A的增加。呼吸道感染C.在鹦鹉热中，我们发现SIM免疫显著降低了感染肺部的细菌负荷和炎症程度，并降低了IFN-γ、TNF-α和IL-6的水平。此外，用CNPs-Ags免疫SIM，对C.结论：以CNP为佐剂的鹦鹉热SIM免疫，可有效地促进鹦鹉热在体内的传播。鹦鹉热Ags可能为开发抗鹦鹉热的疫苗提供一种新的策略。鹦鹉热感染
Background: Chlamydia psittaci is a zoonotic bacteria closely associated with psittacosis/ ornithosis. Vaccination has been recognized as the best way to inhibit the spread of C. psittaci due to the majority ignored of infections. The optimal Chlamydia vaccine was obstructed by the defect of single immunization route and the lack of availability of nontoxic and valid adjuvants.Methods: In this study, we developed a novel immunization strategy, simultaneous (SIM) intramuscular (IM) and intranasal (IN) administration of a C. psittaci antigens (Ags) adjuvanted with chitosan nanoparticles (CNPs). And SIM-CNPs-Ags were used to determine the different types of immune response and the protective role in vivo.Results: CNPs-Ags with zeta-potential values of 13.12 mV and of 276.1 nm showed excellent stability and optimal size for crossing the mucosal barrier with high 71.7% encapsulation efficiency. SIM-CPN-Ags mediated stronger humoral and mucosal responses by producing meaningfully high levels of IgG and secretory IgA (sIgA) antibodies. The SIM route also led to Ags-specific T-cell responses and increased IFN-gamma, IL-2, TNF-alpha and IL-17A in the splenocyte supernatants. Following respiratory infection with C. psittaci, we found that SIM immunization remarkably reduced bacterial load and the degree of inflammation in the infected lungs and made for a lower level of IFN-gamma, TNF-alpha and IL-6. Furthermore, SIM vaccination with CNPs-Ags had obviously inhibited C. psittaci disseminating to various organs in vivo.Conclusion: SIM immunization with CNPs-adjuvanted C. psittaci Ags may present a novel strategy for the development of a vaccine against the C. psittaci infection.