Urinary trypsin inhibitor suppresses surgical stress-facilitated lung metastasis of murine colon 26-L5 carcinoma cells.
Urinary trypsin inhibitor suppresses surgical stress-facilitated lung metastasis of murine colon 26-L5 carcinoma cells.
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发表时间：
2005-03
影响因子：
2
通讯作者：
I. Yoshioka;Yasunori Tsuchiya;Yasushi Aozuka;Y. Onishi;H. Sakurai;K. Koizumi;K. Tsukada;I. Saiki-I.-Saik
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文献类型：
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作者：
I. Yoshioka;Yasunori Tsuchiya;Yasushi Aozuka;Y. Onishi;H. Sakurai;K. Koizumi;K. Tsukada;I. Saiki-I.-Saik
最近，我们报道了手术应激通过诱导蛋白酶如基质金属蛋白酶-9（MMP-9）在肺组织中的表达促进小鼠结肠癌细胞向肺的转移。尿胰蛋白酶抑制剂（UTI）是一种丝氨酸蛋白酶抑制剂，常用于治疗胰腺炎和改善微循环环境。本研究的目的是研究UTI在手术应激诱导的癌症转移的动物模型中的抗转移特性。在将结肠26-L5癌（结肠26-L5）细胞静脉内注射到经受手术应激的小鼠中之后腹膜内施用UTI抑制肺转移的增强（p<0.05）。此外，我们研究了UTI对结肠26-L5中的肿瘤生长、与纤连蛋白的粘附、迁移、侵袭和酶降解的影响。UTI可降低结肠26-L5细胞的侵袭能力和MMP-9对明胶底物的降解。UTI可以提高大手术后癌症患者的治疗效果。
Recently, we have reported that surgical stress promoted the metastasis of murine colon carcinoma cells to the lung by inducing the expression of proteases such as matrix metalloprotease-9 (MMP-9) in lung tissue. Urinary trypsin inhibitor (UTI) is a serine protease inhibitor frequently used to treat pancreatitis and to improve the microcirculatory environment. The purpose of this study was to investigate the anti-metastatic properties of UTI in an animal model of surgical stress-induced cancer metastasis. The intraperitoneal administration of UTI after the intravenous injection of colon 26-L5 carcinoma (colon 26-L5) cells into mice subjected to surgical stress suppressed the enhancement of lung metastasis (p<0.05). Furthermore, we investigated the effect of UTI on tumor growth, adhesion to fibronectin, migration, invasion and enzymatic degradation in colon 26-L5. UTI reduced the invasive ability and the degradation by MMP-9 of gelatin substrate in colon 26-L5 cells. UTI may improve therapeutic efficacy in cancer patients after major surgery.