Infectivity of Immature Neurons to Zika Virus: A Link to Congenital Zika Syndrome
Infectivity of Immature Neurons to Zika Virus: A Link to Congenital Zika Syndrome
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DOI：
10.1016/j.ebiom.2016.06.026
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发表时间：
2016-08-01
期刊：
影响因子：
11.1
通讯作者：
Bagasra, Omar
中科院分区：
文献类型：
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作者：
Hughes, Brandon W.;Addanki, Krishna C.;Bagasra, Omar
背景：流行病学数据强烈表明，巴西的小头畸形病例与寨卡病毒（ZIKV）的持续流行有关。为了进一步巩固可能的联系，我们使用各种神经母细胞瘤（NB）细胞系研究了ZIKV的感染性。将六种未分化的、两种终末分化的和两种视黄酸（RA）诱导的部分分化的细胞系暴露于ZIKV毒株PRVABC 59，其在遗传上类似于法属波利尼西亚毒株，与目前在巴西发现的ZIKV毒株具有97-100%的遗传同源性。所有感染均通过实时PCR（RT-qPCR）、用抗黄病毒E抗体探测的免疫荧光测定（IFA）和细胞病变效应的评价来确认。在六种NB细胞系中的五种中，通过IFA，90%至70%的细胞是阳性的，而对于一种细胞系CCL-127，如通过IFA所测定的，类似于80%的细胞是ZIKV阳性的，但显示出持续感染。两种分化的细胞系JFEN和T-268对ZIKV具有高度抗性，
Background: Epidemiological data strongly suggest that microcephaly cases in Brazil are associated with the ongoing epidemic of Zika virus (ZIKV). In order to further solidify the possible link, we investigated the infectivity of ZIKV using various neuroblastoma (NB) cell lines.Methods: Six undifferentiated, two terminally differentiated and two retinoic acid (RA) -induced, partially differentiated cell lines were exposed to ZIKV strain PRVABC59, which is genetically similar to the French Polynesia strain, with 97-100% genetic homology to the current ZIKV strain found in Brazil. All infections were confirmed by real-time PCR (RT-qPCR), immunofluorescence assay (IFA) probing with anti-flavivirus E antibody, and evaluation of cytopathic effects.Findings: ZIKV infected all six undifferentiated NB cell lines. In five out of six NB cell lines, between 90 and 70% cells were positive by IFA whereas for one cell line, CCL-127, similar to 80% of cells were positive for ZIKV as determined by IFA but showed persistent infection. Two differentiated cell lines, JFEN and T-268, were highly resistant to ZIKV with