The 13C-xylose breath test for the diagnosis of small bowel bacterial overgrowth in children.
The 13C-xylose breath test for the diagnosis of small bowel bacterial overgrowth in children.
复制标题
13C-木糖呼气试验用于诊断儿童小肠细菌过度生长。
DOI：
10.1097/00005176-199708000-00005
复制
发表时间：
1997
影响因子：
2.9
通讯作者：
Heubi,JE
中科院分区：
文献类型：
--
作者：
Dellert,SF;Nowicki,MJ;Farrell,MK;Delente,J;Heubi,JE
BackgroundWe评估的临床效用的13 C-木糖呼吸试验的诊断小肠细菌过度生长的children.MethodsTo确定最佳剂量的13 C-木糖，29名健康儿童，3至12岁，被随机分配到三个剂量的13 C-木糖（10，25，或50毫克）。禁食过夜后，给予口服剂量的13 C-木糖，并每30分钟收集呼吸样品，持续4小时。采用气相色谱-质谱联用技术对样品中的13 CO2进行了分析.使用50毫克的剂量，然后，我们进行了9个呼吸测试，同时十二指肠细菌培养6名儿童，3至12岁，短肠综合征（n= 2），免疫缺陷状态（n= 1），和动力障碍（n= 3）。结果排泄13二氧化碳在呼吸中达到峰值在2.5小时在所有三个对照组。50 mg剂量在每个时间段内产生最高的呼吸中13 CO2排泄中位数峰值和最小范围。6例小肠细菌过度生长（2× 10 5 ~ 3.5× 10 8）患儿的13 CO2峰值均超过对照组相应时间段的最大呼气13 CO2水平（10 0%灵敏度）。在3例阴性培养的患者中，2例呼气试验结果为阴性，1例为阳性（特异性67%）。一个主题有正常化的十二指肠培养和呼吸测试结果抗生素治疗后的小肠细菌overgrowth.ConclusionsOur初步结果表明，剂量为50毫克13 C-木糖，呼吸测试结果可靠地预测小肠细菌过度生长在易感儿童。
BackgroundWe evaluated the clinical utility of the 13 C-xylose breath test for the diagnosis of small bowel bacterial overgrowth in children.MethodsTo determine the optimal dose of 13 C-xylose, 29 healthy children, 3 to 12 years old, were randomly assigned to receive one of three doses of 13 C-xylose (10, 25, or 50 mg). After an overnight fast, the oral dose of 13 C-xylose was administered, and breath samples were collected every 30 minutes for 4 hours. Samples were analyzed for 13 CO 2 by gas chromatography with mass spectrometry. Using the 50 mg dose, we then performed nine breath tests with concurrent duodenal bacterial cultures in 6 children, 3 to 12 years old, with short-bowel syndrome (n= 2), immunodeficiency states (n= 1), and motility disorders (n= 3).ResultsExcretion of 13 CO 2 in breath peaked at 2.5 hours in all three control groups. The 50-mg dose produced the highest median peak and the smallest range of 13 CO 2 excretion in breath within each time period. The time of peak 13 CO 2 excretion in breath varied among the diseased children; however, the six patients with small-bowel bacterial overgrowth (2× 10 5-3.5× 10 8 gram negative rods) all had peak 13 CO 2 that exceeded the maximum breath 13 CO 2 level in breath of the control subjects at the corresponding time period (100% sensitivity). Of the three patients with negative cultures, two had negative breath test results and one had positive results (67% specificity). One subject had normalization of both duodenal culture and breath test results after antibiotic treatment of small-bowel bacterial overgrowth.ConclusionsOur preliminary results suggest that with a dose of 50 mg 13 C-xylose, breath test results reliably predict small-bowel bacterial overgrowth in susceptible children.