LOCALIZATION OF SIALIC-ACID IN KIDNEY GLOMERULI - REGIONALIZATION IN THE PODOCYTE PLASMA-MEMBRANE AND LOSS IN EXPERIMENTAL NEPHROSIS
LOCALIZATION OF SIALIC-ACID IN KIDNEY GLOMERULI - REGIONALIZATION IN THE PODOCYTE PLASMA-MEMBRANE AND LOSS IN EXPERIMENTAL NEPHROSIS
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DOI：
10.1073/pnas.82.24.8508
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发表时间：
1985-12-01
影响因子：
11.1
通讯作者：
ROTH, J
中科院分区：
文献类型：
--
作者：
CHAREST, PM;ROTH, J
Sialic acid residues were localized by electron microscopy in renal glomeruli of normal and puromycin-treated rats with a cytochemical technique that utilized the Limax flavus lectin. In Lowicryl K4M thin sections from normal rats, sialic acid residues were found along the plasma membrane of the various glomerular cell types and in the glomerular basement membrane as well as the mesangial matrix. In NaDodSO4/PAGE, sialic acid residues of normal glomeruli were mainly confined to a 140-kDa protein previously identified as podocalyxin. The distribution of sialic acid residues in the podocyte plasma membrane was found to be remarkably regionalized. Based on the differential labeling intensity, 3 plasma membrane domains could be defined: the foot process base, the foot process region above the slit diaphragm, and the body of podocytes. Cytochemical and biochemical analysis of glomeruli from puromycin-treated rats showed a loss of sialic acid residues from glomerular sialoglycoconjugates indicating a perturbated glycosylation.
