Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots.
Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots.
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DOI：
10.1104/pp.125.1.199
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发表时间：
2001
期刊：
影响因子：
7.4
通讯作者：
Yoko Yamamoto;Yukiko Kobayashi;H. Matsumoto
中科院分区：
文献类型：
--
作者：
Yoko Yamamoto;Yukiko Kobayashi;H. Matsumoto
豌豆（豌豆）根处理铝在钙溶液中，脂质过氧化作用的组织化学和生物化学，以及铝暴露引起的其他事件进行了研究。组织化学染色观察到类似的分布在根尖的整个表面上的三个事件（铝的积累，脂质过氧化和胼胝质的生产），但质膜完整性的损失（检测伊文思蓝摄取）仅局限于根尖表面上的裂缝的周边。四个事件（铝积累，脂质过氧化，胼胝质的生产，根伸长抑制）的增强表现出类似的铝剂量依赖性，并发生在4小时。然而，膜完整性的损失，在较低的铝浓度和较长的铝暴露（8小时）后，增强。在铝处理过程中加入丁基羟基茴香醚（一种亲脂性抗氧化剂）完全阻止了40%的脂质过氧化和胼胝质的产生，但没有阻止或减缓其他事件。因此，脂质过氧化作用是一个相对早期的症状诱导铝的积累，并出现导致，在一定程度上，胼胝质的生产，但不是根伸长抑制;相比之下，质膜完整性的损失是一个相对较晚的症状所造成的裂缝在根由于根伸长的抑制。
Pea (Pisum sativum) roots were treated with aluminum in a calcium solution, and lipid peroxidation was investigated histochemically and biochemically, as well as other events caused by aluminum exposure. Histochemical stainings were observed to distribute similarly on the entire surface of the root apex for three events (aluminum accumulation, lipid peroxidation, and callose production), but the loss of plasma membrane integrity (detected by Evans blue uptake) was localized exclusively at the periphery of the cracks on the surface of root apex. The enhancement of four events (aluminum accumulation, lipid peroxidation, callose production, and root elongation inhibition) displayed similar aluminum dose dependencies and occurred by 4 h. The loss of membrane integrity, however, was enhanced at lower aluminum concentrations and after longer aluminum exposure (8 h). The addition of butylated hydroxyanisole (a lipophilic antioxidant) during aluminum treatment completely prevented lipid peroxidation and callose production by 40%, but did not prevent or slow the other events. Thus lipid peroxidation is a relatively early symptom induced by the accumulation of aluminum and appears to cause, in part, callose production, but not the root elongation inhibition; by comparison, the loss of plasma membrane integrity is a relatively late symptom caused by cracks in the root due to the inhibition of root elongation.