THE CYTOTOXIC EOSINOPHIL CATIONIC PROTEIN (ECP) HAS RIBONUCLEASE-ACTIVITY
THE CYTOTOXIC EOSINOPHIL CATIONIC PROTEIN (ECP) HAS RIBONUCLEASE-ACTIVITY
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DOI：
10.1016/s0006-291x(86)80310-2
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发表时间：
1986-09-30
影响因子：
3.1
通讯作者：
OLSSON, I
中科院分区：
文献类型：
--
作者：
GULLBERG, U;WIDEGREN, B;OLSSON, I
嗜酸性粒细胞阳离子蛋白(ECP)是一种特殊的颗粒细胞毒性成分。在这项工作中，我们证明了ECP具有核糖核酸酶活性。纯化后的ECP经离子交换层析分离得到具有核糖核酸酶活性的亚组分。另一种嗜酸性粒细胞颗粒蛋白EPX与嗜酸性粒细胞衍生神经毒素(EDN)相同，其核糖核酸酶活性是ECP的125倍。ECP对寄生虫和细胞的细胞毒作用可能是因为其极强的碱性，也可能是它内化并通过降解mRNA起作用。
The eosinophil cationic protein (ECP) is a specific cytotoxic constituent of granules. In this work we demonstrated that ECP has a ribonuclease activity. Purified ECP was resolved by ion exchange chromatography into subfractions, which all showed ribonuclease activity. Another eosinophil granule protein, EPX, identical with eosinophil-derived neurotoxin (EDN) had a 125-fold higher RNase activity than ECP. ECP may exert its cytotoxic effects on parasites and cells because of its extreme basicity alone or it may be internalized and act by degrading mRNA.