Redox Regulation of the NOR Transcription Factor Is Involved in the Regulation of Fruit Ripening in Tomato1
Redox Regulation of the NOR Transcription Factor Is Involved in the Regulation of Fruit Ripening in Tomato1
复制标题
NOR 转录因子的氧化还原调控参与番茄果实成熟的调控(1)
DOI：
10.1104/pp.20.00070
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发表时间：
2020-06-01
期刊：
影响因子：
7.4
通讯作者：
Duan, Xuewu
中科院分区：
文献类型：
--
作者：
Jiang, Guoxiang;Zeng, Jing;Duan, Xuewu
转录因子是植物生长发育和逆境应答的重要调节因子。TF本身也倾向于多个翻译后修饰（PTM）。然而，氧化还原介导的PTM的TF在植物中仍然知之甚少。在这里，我们建立了NON-RIPENING（NOR），一个主TF调节番茄（番茄）果实成熟，是一个目标的甲硫氨酸亚砜还原酶A和B，即E4和SlMsrB 2，分别在番茄。NOR中的Met氧化，即磺基氧化，或通过将Met-138突变为Gln来模拟磺基氧化，降低了其DNA结合能力和体外转录调节活性。E4和SlMsrB 2部分修复氧化的NOR并恢复其DNA结合能力。用NOR互补转基因突变体部分挽救了成熟缺陷。然而，与NOR-M138 Q，含有模拟蛋氨酸磺基氧化转化ofNOR，抑制果实成熟表型的恢复，这是与一些成熟相关基因的DNA结合和转录激活减少。两者合计，这些观察结果揭示了一个PTM机制，通过该机制，MSR介导的NOR氧化还原修饰调节成熟相关基因的表达，从而影响番茄果实成熟。我们的报告描述了硫氧化的TF如何调节植物的发育过程。非成熟转录因子的硫氧化通过降低其DNA结合能力和转录调节活性来调节番茄果实的成熟。
Transcription factors (TFs) are important regulators of plant growth and development and responses to stresses. TFs themselves are also prone to multiple posttranslational modifications (PTMs). However, redox-mediated PTM of TFs in plants remains poorly understood. Here, we established that NON-RIPENING (NOR), a master TF regulating tomato (Solanum lycopersicum) fruit ripening, is a target of the Met sulfoxide reductases A and B, namely E4 and SlMsrB2, respectively, in tomato. Met oxidation in NOR, i.e. sulfoxidation, or mimicking sulfoxidation by mutating Met-138 to Gln, reduces its DNA-binding capacity and transcriptional regulatory activity in vitro. E4 and SlMsrB2 partially repair oxidized NOR and restore its DNA-binding capacity. Transgenic complementation of thenormutant with NOR partially rescues the ripening defects. However, transformation ofnorwith NOR-M138Q, containing mimicked Met sulfoxidation, inhibits restoration of the fruit ripening phenotype, and this is associated with the decreased DNA-binding and transcriptional activation of a number of ripening-related genes. Taken together, these observations reveal a PTM mechanism by which Msr-mediated redox modification of NOR regulates the expression of ripening-related genes, thereby influencing tomato fruit ripening. Our report describes how sulfoxidation of TFs regulates developmental processes in plants.Sulfoxidation of NON-RIPENING transcription factor regulates tomato fruit ripening by decreasing its DNA binding capacity and transcriptional regulatory activity.