Protective effects of antioxidin-RL from Odorrana livida against ultraviolet Birradiated skin photoaging
Protective effects of antioxidin-RL from Odorrana livida against ultraviolet Birradiated skin photoaging
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青花香抗氧化剂-RL对紫外线辐射皮肤光老化的保护作用
DOI：
10.1016/j.peptides.2018.01.009
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发表时间：
2018-03-01
期刊：
影响因子：
3
通讯作者：
Gao, Yuanyuan
中科院分区：
文献类型：
--
作者：
Qin, Di;Lee, Wen-Hui;Gao, Yuanyuan
皮肤每天不可避免地暴露于紫外线（UV）B辐射已被证明具有有害影响。抗氧化肽-RL是一种从青蛙Odorrana livida皮肤分泌物中分离纯化的抗氧化肽，其氨基酸序列为AMRLTYNRPCIYAT。为了探讨抗氧化素-RL对UVB辐射皮肤光老化的保护作用，采用细胞摄取法检测了具有多种生物学功能的抗氧化素-RL分子在细胞中的定位。在体外和体内研究了抗氧化剂RL对UVB诱导的反应的保护作用。结果表明，抗氧化剂RL成功地穿透细胞膜，并对细胞迁移产生积极影响。它还能有效地抑制UVB诱导的ROS的过度产生，防止DNA和蛋白质的氧化损伤。此外，MMP-1，VEGF，考克斯-2和促炎细胞因子，如IL-6和TNF-α在抗氧化剂RL处理的HaCaT和HSF细胞中的mRNA表达显著下调，而FGF，I型前胶原和TGF-β 1的mRNA表达上调。抗氧化剂-RL有效地防止UVB诱导的小鼠皮肤红斑，从而抑制UVB诱导的皮肤增厚和炎症，并增加胶原蛋白沉积，如体内实验所示。因此，新的抗氧化肽antioxidin-RL可以有效地减少UVB诱导的皮肤反应在体内和体外，提供潜在的分子对UVB诱导的炎症和光老化。
The unavoidable daily exposure of the skin to ultraviolet (UV) B radiation is proven to have deleterious effects. The action mechanism of antioxidin-RL, an antioxidant peptide purified from skin secretions of frog Odorrana livida with amino acid sequence of AMRLTYNRPCIYAT, is well characterized by NMR titration and mutation based on ABTS(+) scavenging activities. In order to explore the protective effects of antioxidin-RL against UVB-irradiated skin photoaging, cell uptake assay was used to detect the location of antioxidin-RL molecules serving various biological functions in the cells. The protective effects of antioxidin-RL on UVB-induced response were examined in vitro and in vivo. Results showed that antioxidin-RL successfully penetrated the cell membrane and exerted a positive effect on cell migration. It also effectively inhibited the UVB-induced excessive production of ROS and prevented oxidative damage to DNAs and proteins. Moreover, the mRNA expressions of MMP-1, VEGF, COX-2, and pro-inflammatory cytokines, such as IL-6 and TNF-alpha in antioxidin-RL-treated HaCaT and HSF cells were significantly down-regulated whereas those of FGF, procollagen type I and TGF-beta 1 up-regulated. Antioxidin-RL effectively prevented UVB-induced erythema on mouse skin, thereby inhibiting UVB-induced skin thickening and inflammation and increasing collagen deposition as demonstrated by in vivo experiments. Hence, the novel antioxidant peptide antioxidin-RL can effectively reduce UVB-induced skin reactions in vivo and in vitro, providing potential molecules against UVB-induced inflammation and photoaging.