Molecular and Phenotypic Characteristics of CMY-2 β-Lactamase-Producing Salmonella enterica Serovar Typhimurium Isolated from Cattle in Japan
Molecular and Phenotypic Characteristics of CMY-2 β-Lactamase-Producing Salmonella enterica Serovar Typhimurium Isolated from Cattle in Japan
复制标题
DOI：
10.1292/jvms.10-0156
复制
发表时间：
2011-03-01
影响因子：
1.2
通讯作者：
Akiba, Masato
中科院分区：
文献类型：
--
作者：
Sugawara, Masaru;Komori, Junko;Akiba, Masato
对2007年从日本福岛县两个不同养殖场分离的广谱头孢菌素（ESC）耐药肠炎沙门氏菌血清型鼠伤寒沙门氏菌进行了鉴定，以确定耐药的遗传基础。两个分离株的ESC抗性是由bla（CMY-2）基因编码的AmpC β -内酰胺酶介导的，该基因位于每个分离株的一个大的自传质粒中。携带bla（CMY-2）的质粒大小不同。质粒的复制子类型为i - i - γ和A/C。宏观酶切分析和噬菌体分型结果表明，这两个分离株关系密切。这是日本首次报道从牛中分离出具有esc抗性的鼠伤寒沙门氏菌。
Isolates of extended-spectrum cephalosporin (ESC)-resistant Salmonella enterica serovar Typhimurium obtained from two different farms in Fukushima Prefecture, Japan, in 2007 were characterized in order to determine the genetic basis of resistance. ESC resistance in the two isolates was mediated by an AmpC beta-lactamase encoded by the bla(CMY-2) gene, which is located in a large self-transmissible plasmid in each isolate. The sizes of the bla(CMY-2)-carrying plasmids were different. The replicon types of the plasmids were II-I gamma and A/C. The results of macrorestriction analysis and phage typing suggest a close relationship between both isolates. This is the first report of ESC-resistant S. Typhimurium isolated from cattle in Japan.