Potential for Aerobic and Anaerobic Biodegradation of Petroleum Hydrocarbons in Boreal Subsurface
Potential for Aerobic and Anaerobic Biodegradation of Petroleum Hydrocarbons in Boreal Subsurface
复制标题
DOI：
10.1023/b:biod.0000009954.21526.e8
复制
发表时间：
2004-02
期刊：
影响因子：
3.6
通讯作者：
J. Salminen;P. Tuomi;Anna-Mari Suortti;K. Jørgensen
中科院分区：
文献类型：
--
作者：
J. Salminen;P. Tuomi;Anna-Mari Suortti;K. Jørgensen
我们研究了在经历自然衰减的北方轻质燃料和润滑油污染场地中需氧和厌氧石油烃降解的作用。该场地全年缺氧，土壤气体中CH4浓度高达25% v/v，CO2浓度高达18% v/v。地下样品主要取自场地的缺氧部分，它们代表了不饱和区和饱和区。样品在近原位条件（即原位温度 8°C，需氧和厌氧条件，无营养改良剂）下在微观世界中孵育，从而在需氧和厌氧条件下去除矿物油（通过气相色谱法测定）。在有氧微观世界中，在 3 个月和 4 个月的孵化过程中，平均分别去除了 31% 和 27% 的初始矿物油。在厌氧微观世界中，在 12 个月和 10 个月的厌氧培养过程中，平均分别去除了 44% 和 15% 的初始矿物油。除去C11至C15的正烷烃。在这些微观世界中记录到的甲烷生产率高达 2.5 μg CH4h-1g-1dwt。在好氧和厌氧微观世界中，通常90%的降解矿物油属于在C10之后和C15之前从气相色谱中洗脱的矿物油馏分，而10%属于在C15之后和C40之前洗脱的馏分。我们的结果表明，在产甲烷条件下厌氧石油烃降解，包括正烷烃降解，在北方条件下的自然衰减中起着重要作用。
We studied the role of aerobic and anaerobic petroleum hydrocarbon degradation ata boreal, light-weight fuel and lubrication oil contaminated site undergoing naturalattenuation. At the site, anoxic conditions prevailed with high concentrations ofCH4(up to 25% v/v) and CO2(up to 18% v/v) in the soil gas throughout the year. Subsurface samples were obtained mainly from the anoxic parts of the site and they represented both the unsaturated and saturated zone. The samples wereincubated in microcosms at nearin situconditions (i.e.in situtemperature 8 °C, aerobic and anaerobic conditions, no nutrient amendments) resulting in the removal of mineral oil (as determined by gas chromatography) aerobically as well as anaerobically. In the aerobic microcosms on average 31% and 27% of the initial mineral oil was removed during a 3- and 4-month incubation, respectively. In the anaerobic microcosms, on average 44% and 15% of the initial mineral oil was removed during a 12- and 10-month anaerobic incubation, respectively, and e.g. n-alkanes from C11to C15were removed. A methane production rate of up to 2.5 μg CH4h-1g-1dwt was recorded in thesemicrocosms. In the aerobic as well as anaerobic microcosms, typically 90% of themineral oil degraded belonged to the mineral oil fraction that eluted from the gaschromatograph after C10and before C15, while 10% belonged to the fraction that eluted after C15and before C40. Our results suggest that anaerobic petroleum hydrocarbon degradation, including n-alkane degradation, under methanogenic conditions plays a significant role in the natural attenuation in boreal conditions.