A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
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DOI：
10.3389/finsc.2023.1093970
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发表时间：
2023-03
期刊：
影响因子：
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通讯作者：
M. Whitten;Qi Xue;C. N. Taning;Reuben James;G. Smagghe;R. del Sol;M. Hitchings;P. Dyson
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文献类型：
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作者：
M. Whitten;Qi Xue;C. N. Taning;Reuben James;G. Smagghe;R. del Sol;M. Hitchings;P. Dyson
Inhibidal RNAi是一种有针对性的害虫种群控制措施。杀虫RNAi的特异性理论上可以通过使用具有狭窄宿主范围的共生细菌来递送RNAi来增强，这种方法称为共生体介导的RNAi（SMR），我们之前已经在全球入侵害虫物种西方花蓟马（WFT）中证明了这种技术。方法在这里，我们研究的两个主要的细菌共生体的WFT，BFo 1和BFo 2，基因组测序昆虫的分布。此外，我们已经挑战了两个非目标昆虫物种与两种细菌物种，即授粉的欧洲熊蜂，熊蜂terrestris，和昆虫捕食者的WFT，海盗虫Orius laevigatus。结果我们的数据表明，一个非常有限的分布在昆虫之间的共生体以外的WFT。此外，虽然BFo 1可以在蜜蜂和海盗虫中建立自己，尽管对昆虫的适应性没有显着影响，但BFo 2无法在这两个物种中持续存在。讨论在生物安全方面，这些数据，连同其更具体的生长要求，证明选择BFo2用于WFT的RNAi和精确害虫管理。
Introduction Insecticidal RNAi is a targeted pest insect population control measure. The specificity of insecticidal RNAi can theoretically be enhanced by using symbiotic bacteria with a narrow host range to deliver RNAi, an approach termed symbiont-mediated RNAi (SMR), a technology we have previously demonstrated in the globally-invasive pest species Western Flower Thrips (WFT). Methods Here we examine distribution of the two predominant bacterial symbionts of WFT, BFo1 and BFo2, among genome-sequenced insects. Moreover, we have challenged two non-target insect species with both bacterial species, namely the pollinating European bumblebee, Bombus terrestris, and an insect predator of WFT, the pirate bug Orius laevigatus. Results Our data indicate a very limited distribution of either symbiont among insects other than WFT. Moreover, whereas BFo1 could establish itself in both bees and pirate bugs, albeit with no significant effects on insect fitness, BFo2 was unable to persist in either species. Discussion In terms of biosafety, these data, together with its more specific growth requirements, vindicate the choice of BFo2 for delivery of RNAi and precision pest management of WFT.