A comparison of voluntary salt-intake behavior in Na x -gene deficient and wild-type mice with reference to peripheral taste inputs
A comparison of voluntary salt-intake behavior in Na x -gene deficient and wild-type mice with reference to peripheral taste inputs
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DOI：
10.1016/s0006-8993(03)02247-9
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发表时间：
2003-03
期刊：
影响因子：
2.9
通讯作者：
Uno Watanabe;T. Shimura;N. Sako;Jun-ichi Kitagawa;T. Shingai;E. Watanabe;M. Noda;Takashi Yamamoto
中科院分区：
文献类型：
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作者：
Uno Watanabe;T. Shimura;N. Sako;Jun-ichi Kitagawa;T. Shingai;E. Watanabe;M. Noda;Takashi Yamamoto
电压门控钠通道的一个亚家族，被认为是中枢神经系统中的特异性钠受体。我们之前的研究表明，即使在缺水的条件下，Nax基因缺陷小鼠也会摄入过量的NaCl。在本研究中，调查外周味觉输入是否参与了异常摄入的盐在Na X缺陷型小鼠（homo），自愿摄入各种味道的解决方案在homo和野生型小鼠（野生）在非剥夺条件下进行了检查。人表现出更高的偏好0.15 M NaCl溶液比野生的。对其他基本促味剂的偏好比率在组间是相同的。横断鼓索（CT）或舌咽（GP）神经盐摄入行为的影响不大，在人和野生。虽然联合横断的上级喉（SL）和GP神经减少氯化钠的摄入量在同性恋，但不是在野生的，有没有差异的偏好比例氯化钠在同性恋SL+GP横断之前和之后。另一方面，在野生的NaCl的偏好比率趋于增加后，结合SL和GP横断。因此，SL+GP横切后，对NaCl的偏好比率在人与野生之间没有差异。虽然电生理反应的CT和GP的各种口味的解决方案之间的同性恋和野生的区别，那些SL的NaCl在同性恋小于野生的只有在较低的浓度（0.01和0.03 M）。因此，从口咽部区域的化学感受输入对异常盐摄入量的影响很小，如果有的话。从这些结果，这表明，较高的偏好NaCl在人主要是由于缺乏Na+通道在中枢神经系统。
The Naxchannel, a subfamily of voltage-gated sodium channels, is thought to be a specific sodium receptor in the central nervous system. Our previous study revealed that Nax-gene-deficient mice consumed excessive amounts of NaCl even under water-deprived conditions. In the present study, to investigate whether the peripheral taste inputs are involved in the abnormal intake of salt in Nax-deficient mice (homo), voluntary intake of various taste solutions in homo and wild-type mice (wild) was examined under non-deprived conditions. Homo showed a higher preference for 0.15 M NaCl solution than wild. Preference ratios for other basic tastants were identical between groups. Transection of the chorda tympani (CT) or the glossopharyngeal (GP) nerve had little effect on salt-intake behavior in homo and wild. Although combined transection of the superior laryngeal (SL) and GP nerves decreased NaCl intake in homo but not in wild, there were no differences in preference ratios for NaCl in homo before and after SL+GP transection. On the other hand, preference ratios for NaCl in wild tended to increase after combined SL and GP transection. Consequently, preference ratios for NaCl after SL+GP transection were no different between homo and wild. While electrophysiological responses of the CT and the GP to various taste solutions were indistinguishable between homo and wild, those of the SL to NaCl in homo were smaller than those in wild only at lower concentrations (0.01 and 0.03 M). Thus, chemosensory inputs from the oro-pharyngeal regions had little effect on abnormal salt intake in homo, if any. From these results, it is suggested that the higher preference for NaCl in homo is mainly due to the lack of Naxchannels in the central nervous system.