Gene body-specific methylation on the active X chromosome
Gene body-specific methylation on the active X chromosome
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DOI：
10.1126/science.1136352
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发表时间：
2007-02-23
期刊：
影响因子：
56.9
通讯作者：
Chess, Andrew
中科院分区：
文献类型：
--
作者：
Hellman, Asaf;Chess, Andrew
差异DNA甲基化对于基因表达的表观遗传调控是重要的。在启动子CpG岛上已经证实了非活性X染色体的等位基因特异性甲基化，但活性X(Xa)和非活性X(Xi)染色体上甲基化的总体模式尚不清楚。我们对人类X染色体上1000多个信息位点进行了等位基因特异性分析。Xa显示的等位基因特异性甲基化是Xi的两倍多。这种甲基化集中在基因体，影响多个相邻的CPG。在X失活之前，所有这些Xa基因的体甲基化位点都是双等位甲基化的。因此，两部分甲基化-去甲基化程序导致基因启动子上Xa特异性的低甲基化和基因体上的高甲基化。这些结果提示了全局甲基化与表达潜力之间的关系。
Differential DNA methylation is important for the epigenetic regulation of gene expression. Allele-specific methylation of the inactive X chromosome has been demonstrated at promoter CpG islands, but the overall pattern of methylation on the active X (Xa) and inactive X ( Xi) chromosomes is unknown. We performed allele-specific analysis of more than 1000 informative loci along the human X chromosome. The Xa displays more than two times as much allele-specific methylation as Xi. This methylation is concentrated at gene bodies, affecting multiple neighboring CpGs. Before X inactivation, all of these Xa gene body-methylated sites are biallelically methylated. Thus, a bipartite methylation-demethylation program results in Xa-specific hypomethylation at gene promoters and hypermethylation at gene bodies. These results suggest a relationship between global methylation and expression potentiality.