Effect of Salinity Stress on Enzymes' Activity, Ions Concentration, Oxidative Stress Parameters, Biochemical Traits, Content of Sulforaphane, and CYP79F1 Gene Expression Level in Lepidium draba Plant
Effect of Salinity Stress on Enzymes' Activity, Ions Concentration, Oxidative Stress Parameters, Biochemical Traits, Content of Sulforaphane, and CYP79F1 Gene Expression Level in Lepidium draba Plant
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DOI：
10.1007/s00344-019-10047-6
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发表时间：
2019-11-06
影响因子：
4.8
通讯作者：
Ahsaei, Mahshid Ghazizadeh
中科院分区：
文献类型：
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作者：
Goharrizi, Kiarash Jamshidi;Riahi-Madvar, Ali;Ahsaei, Mahshid Ghazizadeh
在本研究的第一步中，研究了50 mM NaCl对五种不同的独行菜种子发芽率的影响。draba）生态型，其中Rafsanjan生态型的种子发芽率最高。第二步，以14日龄的红腹锦鸡儿为材料，对其生理生化、形态学、细胞色素P450 79 F1（CYP 79 F1）的表达水平以及萝卜硫素（SFN）含量等沿着变化进行了研究。结果表明，随着NaCl浓度的增加，Rafsanjan生态型种子的发芽率沿着茎长和根长呈下降趋势。抗坏血酸过氧化物酶，愈创木酚过氧化物酶和超氧化物歧化酶的活性增加到75 mM NaCl，然后下降。随着NaCl浓度的增加，Na+和Cl-浓度增加，而P，Ca ~（2+）和K ~+浓度降低。此外，积累的一些氧化应激参数，包括电解质泄漏，丙二醛，其他醛类，和过氧化氢增加，增加在所有样品中的NaCl浓度。此外，总酚、总黄酮、总花色素苷、总游离氨基酸和总可溶性碳水化合物的含量随着NaCl浓度的增加而增加。在本研究中，SFN形成随着氯化钠浓度的增加而增加至75 mM，并且在更高浓度下减少。在最后一步中，鉴定了部分CYP 79 F1 mRNA及其蛋白质序列并在GenBank中注册，然后计算在0、25、50、75和100 mM NaCl下CYP 79 F1基因表达水平的变化。CYP 79 F1的基因表达水平也显示出与盐胁迫下SFN形成相同的模式。
In the first step in this study, the effect of 50 mM NaCl was studied on germination percentage of five different Lepidium draba (L. draba) ecotypes, and Rafsanjan ecotype was selected as experimental material as it had the highest germination percentage. In the second step, some biochemical, physiological, and morphological traits along with content of sulforaphane (SFN) as well as the expression level of Cytochorome P450 79F1 (CYP79F1) were evaluated in 14-day-old L. draba sprouts that grew 9 days in the presence of various concentrations of NaCl including 0, 25, 50, 75, and 100 mM. According to the results of this study, germination percentage of Rafsanjan ecotype along with lengths of stem and root were declined with increasing concentrations of NaCl. Ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase enzymes activity increased up to 75 mM NaCl and then decreased. With increasing the doses of NaCl, concentrations of Na+ and Cl- increased, whereas P, Ca2+, and K+ decreased. Also, accumulation of some oxidative stress parameters including electrolyte leakage, malondialdehyde, other aldehydes, and hydrogen peroxide increased with increasing NaCl concentrations in all samples. Furthermore, contents of total phenolic, total flavonoid, total anthocyanin, total free amino acids, and total soluble carbohydrate were induced with the induction of NaCl concentrations. In this study, SFN formation increased with increasing concentration of sodium chloride up to 75 mM and decreased at higher concentration. In the last step, a partial CYP79F1 mRNA and its protein sequence were identified and registered in GenBank and then changes in the CYP79F1 gene expression levels under 0, 25, 50, 75, and 100 mM NaCl were calculated. The gene expression levels of CYP79F1 also showed the same pattern as was seen for SFN formation under salinity stress.