Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering
Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering
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DOI：
10.1016/j.biomaterials.2011.05.065
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发表时间：
2011-10-01
期刊：
影响因子：
14
通讯作者：
Weiss, Anthony S.
中科院分区：
文献类型：
--
作者：
Rnjak-Kovacina, Jelena;Wise, Steven G.;Weiss, Anthony S.
采用低（1 mL/h）流速和高（3 mL/h）流速进行静电纺丝，获得了低孔隙率和高孔隙率的合成人弹性蛋白支架。对这些支架的物理、力学和生物学性能进行了筛选，以确定真皮组织生物工程的最佳候选材料。高流速电纺丝SHE支架纤维直径增大，平均孔径增大，支架整体孔隙率增加一倍以上。两种支架的杨氏模量与天然弹性蛋白相当，但高孔隙率支架具有更高的抗拉强度。低孔隙率和高孔隙率支架支持原代真皮成纤维细胞早期附着、扩散和增殖，但只有高孔隙率支架支持细胞主动迁移和浸润到支架内。高孔隙率SHE支架促进了细胞的持久性和支架的体外重塑，只有适度的支架收缩。在一项小鼠皮下植入研究中，支架在表面和浸润的成纤维细胞中持续存在至少6周，这是支架重塑的证据，包括从头胶原合成和早期血管生成。(C) 2011 Elsevier Ltd.版权所有。
We obtained low and high porosity synthetic human elastin scaffolds by adapting low (1 mL/h) and high (3 mL/h) flow rates respectively during electrospinning. Physical, mechanical and biological properties of these scaffolds were screened to identify the best candidates for the bioengineering of dermal tissue. SHE scaffolds that were electrospun at the higher flow rate presented increased fiber diameter and greater average pore size and over doubling of overall scaffold porosity. Both types of scaffold displayed Young's moduli comparable to that of native elastin, but the high porosity scaffolds possessed higher tensile strength. Low and high porosity scaffolds supported early attachment, spreading and proliferation of primary dermal fibroblasts, but only high porosity scaffolds supported active cell migration and infiltration into the scaffold. High porosity SHE scaffolds promoted cell persistence and scaffold remodeling in vitro with only moderate scaffold contraction. The scaffolds persisted for at least 6 weeks in a mouse subcutaneous implantation study with fibroblasts on the exterior and infiltrating, evidence of scaffold remodeling including de novo collagen synthesis and early stage angiogenesis. (C) 2011 Elsevier Ltd. All rights reserved.