Pax6 Lengthens G1 Phase and Decreases Oscillating Cdk6 Levels in Murine Embryonic Cortical Progenitors.
Pax6 Lengthens G1 Phase and Decreases Oscillating Cdk6 Levels in Murine Embryonic Cortical Progenitors.
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DOI：
10.3389/fncel.2018.00419
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发表时间：
2018
影响因子：
5.3
通讯作者：
Price DJ
中科院分区：
文献类型：
--
作者：
Mi D;Manuel M;Huang YT;Mason JO;Price DJ
Pax6是大脑皮质发生中祖细胞分裂速率的关键调节因子。先前的研究表明，这种作用至少部分是由细胞周期基因Cdk6的调控介导的，Cdk6主要作用于细胞从G1期向S期的转变。我们通过调查除Cdk6外，其他Pax6调节的细胞周期基因是否可能是Pax6对皮质祖细胞周期作用的主要介质开始了本研究。在Pax6的急性皮质特异性缺失后，Cdk6的表达变化比任何其他Pax6调节的细胞周期基因早一天，这表明它是Pax6作用的主要介质。然后我们用流式细胞术显示缺乏Pax6的祖细胞G1期缩短，并且它们的Cdk6水平在所有期都增加。我们发现Cdk6水平在细胞周期中振荡，从G1期增加到M期。我们提出了一个模型，其中Pax6的缺失通过提高整体Cdk6水平缩短G1期，从而缩短Cdk6水平跨越触发过渡到S期的阈值所需的时间。
Pax6 is a key regulator of the rates of progenitor cell division in cerebral corticogenesis. Previous work has suggested that this action is mediated at least in part by regulation of the cell cycle gene Cdk6, which acts largely on the transition from G1 to S phase. We began the present study by investigating whether, in addition to Cdk6, other Pax6-regulated cell cycle genes are likely to be primary mediators of Pax6’s actions on cortical progenitor cell cycles. Following acute cortex-specific deletion of Pax6, Cdk6 showed changes in expression a day earlier than any other Pax6-regulated cell cycle gene suggesting that it is the primary mediator of Pax6’s actions. We then used flow cytometry to show that progenitors lacking Pax6 have a shortened G1 phase and that their Cdk6 levels are increased in all phases. We found that Cdk6 levels oscillate during the cell cycle, increasing from G1 to M phase. We propose a model in which loss of Pax6 shortens G1 phase by raising overall Cdk6 levels, thereby shortening the time taken for Cdk6 levels to cross a threshold triggering transition to S phase.
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影响因子：
16.2
作者：
Mi D;Carr CB;Georgala PA;Huang YT;Manuel MN;Jeanes E;Niisato E;Sansom SN;Livesey FJ;Theil T;Hasenpusch-Theil K;Simpson TI;Mason JO;Price DJ
通讯作者：
Price DJ
DOI：
10.1242/dev.02764
发表时间：
2007-02
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Manuel M;Georgala PA;Carr CB;Chanas S;Kleinjan DA;Martynoga B;Mason JO;Molinek M;Pinson J;Pratt T;Quinn JC;Simpson TI;Tyas DA;van Heyningen V;West JD;Price DJ
通讯作者：
Price DJ
影响因子：
64.8
作者：
HILL, RE;FAVOR, J;VANHEYNINGEN, V
通讯作者：
VANHEYNINGEN, V
影响因子：
2.7
作者：
Martynoga, B;Morrison, H;Mason, JO
通讯作者：
Mason, JO
影响因子：
5.3
作者：
Manuel MN;Mi D;Mason JO;Price DJ
通讯作者：
Price DJ