Reliability of the E-test method for detection of colistin resistance in clinical isolates of Acinetobacter baumannii
Reliability of the E-test method for detection of colistin resistance in clinical isolates of Acinetobacter baumannii
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DOI：
10.1128/jcm.43.2.903-905.2005
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发表时间：
2005-02-01
影响因子：
9.4
通讯作者：
Aznar, J
中科院分区：
文献类型：
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作者：
Arroyo, LA;García-Curiel, A;Aznar, J
我们比较了E-test和微量肉汤稀释法检测115株鲍曼不动杆菌对粘菌素的敏感性。参考肉汤微量稀释法测定22株（19.1%）对粘菌素耐药，93株（80.8%）敏感。发现98.2%的绝对一致性，只有两个（1.7%）非常重大的错误。E-试验和肉汤微量稀释法在1倍稀释内的一致性为16.5%。MIC在0.25 - 1 μ g粘菌素/ml范围内的菌株完全一致。然而，一致性较差，特别是在通过E-检验方法具有较高MIC的极端稀释度中。
We compared the E-test to the broth microdilution method for testing the susceptibility of 115 clinical isolates of Acinetobacter baumannii to colistin. Twenty-two (19.1%) strains were resistant to colistin and 93 (80.8%) strains were susceptible according to the reference broth microdilution method. A categorical agreement of 98.2% was found, with only two (1.7%) very major errors. Agreement within I twofold dilution between the E-test and the broth microdilution was 16.5%. Complete agreement was found for the strains for which MICs fell within the range of 0.25 to 1 mug of colistin/ml. However, there was poor concordance, particularly in extreme dilutions with higher MICs by the E-test method.