Oxysterols in cosmetics-Determination by planar solid phase extraction and gas chromatography-mass spectrometry.
Oxysterols in cosmetics-Determination by planar solid phase extraction and gas chromatography-mass spectrometry.
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DOI：
10.1016/j.chroma.2016.10.056
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发表时间：
2016-11
期刊：
影响因子：
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通讯作者：
S. Schrack;C. Hohl;W. Schwack
中科院分区：
文献类型：
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作者：
S. Schrack;C. Hohl;W. Schwack
甾醇氧化产物(SOP)与几种毒理作用有关。因此，研究潜在的膳食摄取来源，特别是动物来源的食物，一直是这些化合物的关键问题。为了同时测定复杂化妆品基质中胆固醇、植物甾醇、二氢羊毛甾醇和羊毛甾醇中的氧化甾醇，采用平面固相萃取(PSPE)作为净化工具。用正相薄层层析法将SOP从较多的非极性和极性基质组分中分离出来，然后聚焦到一个靶区。用薄层层析-质谱仪进行区域提取，然后用气相色谱-质谱仪进行分析。PSPE对化妆品样品的净化效果良好，样品萃取物无干扰，色谱柱未见超载迹象。回收率在86%~113%之间，相对标准偏差小于10%(n=6)。我们在2016年的市场调查结果显示，一些含有植物成分的化妆品含有低ppm的植物类固醇氧化产物(POP)，因此与食品报告的水平一致。在含有羊毛脂的产品中，总SOPs水平(胆固醇氧化产物(COPS)、羊毛甾醇氧化产物(LOP)、二氢羊毛甾醇氧化产物(DOPS))处于低百分比范围，比报告的食品水平高出几个数量级。
Sterol oxidation products (SOPs) are linked to several toxicological effects. Therefore, investigation of potential dietary uptake sources particularly food of animal origin has been a key issue for these compounds. For the simultaneous determination of oxysterols from cholesterol, phytosterols, dihydrolanosterol and lanosterol in complex cosmetic matrices, planar solid phase extraction (pSPE) was applied as clean-up tool. SOPs were first separated from more non-polar and polar matrix constituents by normal phase thin-layer chromatography and then focussed into one target zone. Zone extraction was performed with the TLC–MS interface, followed by gas chromatography-mass spectrometry analysis. pSPE showed to be effective for cleaning up cosmetic samples as sample extracts were free of interferences, and gas chromatographic columns did not show any signs of overloading. Recoveries were between 86 and 113% with relative standard deviations of below 10% (n = 6). Results of our market survey in 2016 showed that some cosmetics with ingredients of plant origin contained phytosterol oxidation products (POPs) in the low ppm range and therefore in line with levels reported for food. In lanolin containing products, total SOPs levels (cholesterol oxidation products (COPs), lanosterol oxidation products (LOPs), dihydrolanosterol oxidation products (DOPs)) being in the low percent range exceeded reported levels for food by several orders of magnitudes.