Protection of Grouper Against Cryptocaryon irritans by Immunization With Tetrahymena thermophila and Protective Cross-Reactive Antigen Identification.
Protection of Grouper Against Cryptocaryon irritans by Immunization With Tetrahymena thermophila and Protective Cross-Reactive Antigen Identification.
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通过嗜热四膜虫免疫和保护性交叉反应抗原鉴定来保护石斑鱼免受白点虫刺激
DOI：
10.3389/fimmu.2022.891643
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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接种疫苗是预防刺激隐核虫感染的有效方法。虽然已经开发出一些疫苗，但这些疫苗的大规模生产成本高昂。开发由低成本抗原产生的异种疫苗是一种替代方法。在本研究中，用嗜热四膜虫（一种易在廉价培养基中以高密度生长的自由生活的纤毛虫）免疫石斑鱼，对C.刺激物感染。对C. irritans theronts在T.嗜热菌免疫石斑鱼血清，这表明在血清中存在交叉反应性抗体。通过免疫沉淀和质谱分析，微管蛋白被鉴定为C. irritans和T.嗜热菌重组T.制备了嗜热芽孢杆菌微管蛋白（rTt-tubulin）及其抗体，免疫荧光检测表明，两者均能在大肠杆菌中表达。irritans和T.用抗rTt-微管蛋白抗体对嗜热菌纤毛进行染色。用重组Tt-微管蛋白免疫石斑鱼后，感染率降低。刺激物挑战。C水平的提高。用rTt-微管蛋白免疫石斑鱼，在血清中检测到刺激素结合免疫球蛋白M（IgM）抗体。此外，在rTt-微管蛋白免疫的石斑鱼的粘液和组织培养液中也发现了特异性抗体。总的来说，这些结果表明，接种T。石斑鱼对嗜热菌C. irritans和T.嗜热菌可能是一种潜在的疫苗开发的异源抗原。
Vaccination is an effective method to prevent Cryptocaryon irritans infection. Although some vaccines have been developed, large-scale production of these vaccines is costly. Development of a heterogenous vaccine generated by low-cost antigens is an alternative method. In the present study, grouper immunized with Tetrahymena thermophila, a free-living ciliate that easily grows in inexpensive culture media at high density, showed protective immunity against C. irritans infection. Higher immobilization against C. irritans theronts was detected in T. thermophila–immunized grouper serum, which suggested the existence of a cross-reactive antibody in the serum. By immunoprecipitation and mass spectrometry analyses, tubulin was identified as a potential cross-reactive antigen between C. irritans and T. thermophila. Recombinant T. thermophila tubulin protein (rTt-tubulin) and its antibody were prepared, and immunofluorescence showed that both C. irritans and T. thermophila cilia were stained by the anti–rTt-tubulin antibody. Grouper immunized with rTt-tubulin showed a reduced infective rate after the C. irritans challenge. An enhanced level of C. irritans–binding immunoglobulin M (IgM) antibody was detected in serum from rTt-tubulin–immunized grouper. Moreover, specific antibodies were also found in the mucus and tissue culture medium from rTt-tubulin–immunized grouper. Overall, these findings suggested that vaccination with T. thermophila elicits cross-reactive protective immunity in grouper against C. irritans, and T. thermophila may be a potential heterologous antigen for vaccine development.