COMPARISON OF MB-CHECK, BACTEC, AND EGG-BASED MEDIA FOR RECOVERY OF MYCOBACTERIA
COMPARISON OF MB-CHECK, BACTEC, AND EGG-BASED MEDIA FOR RECOVERY OF MYCOBACTERIA
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DOI：
10.1128/jcm.30.4.878-881.1992
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发表时间：
1992-04-01
影响因子：
9.4
通讯作者：
MORI, T
中科院分区：
文献类型：
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作者：
ABE, C;HOSOJIMA, S;MORI, T
对两相(MB-Check；日本罗氏株式会社，日本东京)和放射学(BACTEC；日本贝顿迪金森有限公司，日本东京)液体培养系统和鸡蛋培养基(3%Ogawa和Ogawa K)的回收率和从临床标本中检测到分枝杆菌的时间进行了测量。在245份痰标本中，共检出分支杆菌86株(35.1%)。其中MB-Check和BACTEC系统分别检出81株(94.2%)和80株(93.0%)，3%Ogawa卵法检出65株(75.6%)。液体培养法和3%小川卵法的阳性培养百分率差异有统计学意义(P<；0.01)。这一差异在涂片阴性的标本中甚至更大。液基系统的检测时间较短。MB-Check系统、BACTEC系统和3%Ogawa卵子法检测结核分枝杆菌复合体的平均时间分别为19.1d、13.4d和21.7d。这些结果表明，MB-CHECK和BACTEC两种基于液体介质的系统都能有效地回收分枝杆菌。
The rate of recovery and time to the detection of mycobacteria from clinical specimens were measured for biphasic (MB-Check; Nippon Roche Co., Ltd., Tokyo, Japan) and radiometric (BACTEC; Nippon Becton Dickinson Co., Ltd., Tokyo, Japan) liquid-based culture systems and egg-based media (3% Ogawa and Ogawa K). From the 245 sputum specimens processed, a total of 86 (35.1%) mycobacterial isolates were detected. Of these, 81 (94.2%) and 80 (93.0%) isolates were detected with the MB-Check and BACTEC systems, respectively, and 65 (75.6%) isolates were detected with the 3% Ogawa egg method. The difference in the percentages of positive cultures between the two systems based on liquid media and the 3% Ogawa egg method was significant (P < 0.01). This difference was even greater among smear-negative specimens. The detection time was shorter with the liquid-based systems. The mean times to the detection of the Mycobacterium tuberculosis complex were 19.1 days with the MB-Check system, 13.4 days with the BACTEC system, and 21.7 days with the 3% Ogawa egg method. These results indicate that both the MB-Check and the BACTEC systems, based on liquid media, are efficient for the recovery of mycobacteria.