A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
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DOI：
10.1038/nnano.2006.206
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发表时间：
2007-02-01
影响因子：
38.3
通讯作者：
Han, Jongyoon
中科院分区：
文献类型：
--
作者：
Fu, Jianping;Schoch, Reto B.;Han, Jongyoon
微制造的规则筛分结构作为凝胶的替代品，在提高生物分子分离的速度和分辨率方面具有很大的前景。与无序的多孔凝胶网络相反，这些规则的结构也为在封闭空间中研究分子动力学提供了良好的环境。然而，迄今为止，常规筛分结构的使用仅限于长DNA分子的分离，然而较小的生理相关大分子（例如蛋白质）的分离仍然是一个挑战。在这里，我们报告了一个由二维周期性纳米流体过滤器阵列组成的微制造各向异性筛分结构。设计的结构各向异性导致不同大小或带电荷的生物分子遵循不同的轨迹，从而实现有效的分离。实现了DNA和蛋白质的连续流基于尺寸的分离，以及蛋白质的静电分离，证明了该装置作为用于集成生物分子样品制备和分析系统的通用分子筛结构的潜在用途。
Microfabricated regular sieving structures hold great promise as an alternative to gels to improve the speed and resolution of biomolecule separation. In contrast to disordered porous gel networks, these regular structures also provide well defined environments ideal for the study of molecular dynamics in confining spaces. However, the use of regular sieving structures has, to date, been limited to the separation of long DNA molecules, however separation of smaller, physiologically relevant macromolecules, such as proteins, still remains a challenge. Here we report a microfabricated anisotropic sieving structure consisting of a two-dimensional periodic nanofluidic filter array. The designed structural anisotropy causes different-sized or -charged biomolecules to follow distinct trajectories, leading to efficient separation. Continuous-flow size-based separation of DNA and proteins, as well as electrostatic separation of proteins, was achieved, demonstrating the potential use of this device as a generic molecular sieving structure for an integrated biomolecule sample preparation and analysis system.