Recordings in an integrating central neuron provide a quick way for identifying appropriate anaesthetic use in fish
Recordings in an integrating central neuron provide a quick way for identifying appropriate anaesthetic use in fish
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DOI：
10.1038/s41598-018-36130-8
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发表时间：
2018-12-03
影响因子：
4.6
通讯作者：
Schuster, Stefan
中科院分区：
文献类型：
--
作者：
Machnik, Peter;Schirmer, Elisabeth;Schuster, Stefan
在畜牧业、畜牧业和研究机构中，麻醉药经常用于缓解压力干预。对于哺乳动物和鸟类，已经建立了根据干预措施微调麻醉的程序。然而，在恒温脊椎动物中，尽管立法发生了变化，关于它们认知能力的证据也越来越多，但目前可用的信息还不足以做出类似的明智决定。在这里，我们建议一种直接的方法来快速填补这一空白。通过记录鱼类大脑中的一个指令神经元（其关键作用是整合和处理来自所有感觉系统的信息并将其传递到运动输出通路），可以直接有效地探索候选麻醉对感觉信息中央处理的特定影响。我们的方法提供了一种快速可靠的方法，可以确定给定麻醉剂是否以及在何种浓度下影响中枢神经系统和感觉处理。我们将我们的方法应用于四种常用的鱼类麻醉药，并证明我们的方法快速和少量的动物为麻醉药使用的知情决策提供了关键数据。
In animal husbandry, livestock industry and research facilities, anaesthetic agents are frequently used to moderate stressful intervention. For mammals and birds, procedures have been established to fine-tune anaesthesia according to the intervention. In ectothermic vertebrates, however, and despite changes in legislation and growing evidence on their cognitive abilities, the presently available information is insufficient to make similarly informed decisions. Here we suggest a straightforward way for rapidly filling this gap. By recording from a command neuron in the brain of fish whose crucial role requires it to integrate and process information from all sensory systems and to relay it to motor output pathways, the specific effects of candidate anaesthesia on central processing of sensory information can directly and efficiently be probed. Our approach allows a rapid and reliable way of deciding if and at which concentration a given anaesthetic affects the central nervous system and sensory processing. We employ our method to four anaesthetics commonly used in fish and demonstrate that our method quickly and with small numbers of animals provides the critical data for informed decisions on anaesthetic use.