A CsgD-independent pathway for cellulose production and biofilm formation in Escherichia coli
A CsgD-independent pathway for cellulose production and biofilm formation in Escherichia coli
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DOI：
10.1128/jb.188.8.3073-3087.2006
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发表时间：
2006-04-01
影响因子：
3.2
通讯作者：
Ghigo, JM
中科院分区：
文献类型：
--
作者：
Da Re, S;Ghigo, JM
表面上的细菌生长通常涉及富含多糖的细胞外基质的产生，其为生物膜群落的形成提供结构支持。在沙门氏菌中，纤维素是生物膜基质的主要成分之一。它的产生受CsgD和在转录后水平激活纤维素合成的二鸟苷酸环化酶AdrA调节。在这里，我们研究了一组大肠杆菌分离株，我们发现，生产纤维素的能力是超过50%的测试菌株共有的共同特征。我们研究了纤维素生产的遗传决定因素及其在生物膜形成中的作用。coli1094。与沙门氏菌的纤维素调节级联相反，在大肠杆菌中既不需要CsgD也不需要AdrA。coli 1094来调节纤维素的产生。在该菌株中，使用替代纤维素调节途径，其涉及GGDEF结构域蛋白YedQ。尽管AdrA（1094）具有功能，但它在大肠杆菌中表达较弱。coli 1094相比，YedQ在所有测试的环境条件下组成型激活纤维素生产。对其它几种E.结果表明，除CsgD/AdrA调节途径外，还存在CsgD非依赖性/YedQ非依赖性和CsgD非依赖性/YedQ非依赖性两种途径，表明纤维素途径在大肠杆菌中普遍存在。大肠杆菌中，并可能在其他产纤维素的肠杆菌科中。
Bacterial growth on a surface often involves the production of a polysaccharide-rich extracellular matrix that provides structural support for the formation of biofilm communities. In Salmonella, cellulose is one of the major constituents of the biofilm matrix. Its production is regulated by CsgD and the diguanylate cyclase AdrA that activates cellulose synthesis at a posttranscriptional level. Here, we studied a collection of Escherichia coli isolates, and we found that the ability to produce cellulose is a common trait shared by more than 50% of the tested strains. We investigated the genetic determinants of cellulose production and its role in biofilm formation in the commensal strain E. coli 1094. By contrast with the Salmonella cellulose regulatory cascade, neither CsgD nor AdrA is required in E. coli 1094 to regulate cellulose production. In this strain, an alternative cellulose regulatory pathway is used, which involves the GGDEF domain protein, YedQ. Although AdrA(1094) is functional, it is weakly expressed in E. coli 1094 compared to YedQ, which constitutively activates cellulose production under all tested environmental conditions. The study of cellulose regulation in several other E. coli isolates showed that, besides the CsgD/AdrA regulatory pathway, both CsgD-independent/YedQ-dependent and CsgD-independent/YedQ-independent pathways are found, indicating that alternative cellulose pathways are common in E. coli and possibly in other cellulose-producing Enterobacteriaceae.