Detection of biofilm formation in Staphylococcus epidermidis from implant infections.: Comparison of a PCR-method that recognizes the presence of ica genes with two classic phenotypic methods
Detection of biofilm formation in Staphylococcus epidermidis from implant infections.: Comparison of a PCR-method that recognizes the presence of ica genes with two classic phenotypic methods
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DOI：
10.1002/jbm.a.30552
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发表时间：
2006-02-01
影响因子：
4.9
通讯作者：
Montanaro, L
中科院分区：
文献类型：
--
作者：
Arciola, CR;Campoccia, D;Montanaro, L
生物膜形成能力越来越被认为是表皮葡萄球菌的一个重要毒力因子。本研究比较了三种不同的检测生物膜阳性菌株的技术。采用PCR方法对80株骨科种植体感染的表皮葡萄球菌进行了生物膜合成基因icaA和icaD的检测。将分子分析结果与刚果红琼脂（CRA）平板法和微滴板法（MtP）两种经典表型方法进行比较。在所有被检测的菌株中，57%的菌株被发现为icaA/ icad阳性，其中只有3个菌株的CRA测试不阳性。不同的是，通过MtP方法，66%的菌株被发现是生物膜生产者，但由于观察到（icaA/icaD+）MtP-菌株（8%）和（icaA/icaD-）MtP+菌株（16%）的结果令人惊讶地模糊，因此与pcr方法的一致性很有限。弱生物膜产生者对这些不匹配结果的贡献最大（10%）。CRA平板检测与ica基因分子检测的一致性较好，表明前者是临床分离株毒力表型表征的可靠方法。然而，MtP方法仍然是使用参考菌株体外筛选不同生物材料粘附性能的宝贵工具。(c) 2005 Wiley期刊有限公司
Biofilm-forming ability is increasingly being recognized as an important virulence factor in Staphylococcus epidermidis. This study compares three different techniques for the detection of biofilm-positive strains. The presence of icaA and icaD genes responsible for biofilm synthesis was investigated by a PCR method in a collection of 80 S. epidermidis strains isolated from orthopedic implant infections. The results from molecular analysis were compared with those obtained by two classic phenotypic methods, the Congo red agar (CRA) plate test and the microtiter plate test (MtP). Fifty-seven percent of all the examined strains were found icaA/icaD-positive, of which only three were not positive for CRA test. Differently, by the MtP method, 66% of the strains were found to be biofilm-producers but only a limited agreement with the PCR-method was noticeable be-cause of the observation of (icaA/icaD+)MtP- strains (8%) and of a surprising ambiguous result of (icaA/icaD-)MtP+ strains (16%). The category of the weak biofilm-producers provided the highest contribution to these mismatching results (10%). The better agreement between the CRA plate test with the molecular detection of ica genes indicates the former as a reliable test for the phenotypic characterization of virulence of clinical isolates. However, MtP method remains a precious tool for the in vitro screening of different biornaterials for the adhesive properties using a reference strain. (c) 2005 Wiley Periodicals, Inc.