REDUCTION OF THIAZINE DYES BY BOVINE PULMONARY ARTERIAL ENDOTHELIAL-CELLS IN CULTURE
REDUCTION OF THIAZINE DYES BY BOVINE PULMONARY ARTERIAL ENDOTHELIAL-CELLS IN CULTURE
复制标题
DOI：
10.1152/ajplung.1995.269.1.l78
复制
发表时间：
1995-07-01
影响因子：
4.9
通讯作者：
DAWSON, CA
中科院分区：
文献类型：
--
作者：
BONGARD, RD;MERKER, MP;DAWSON, CA
将这些噻嗪染料加入到细胞周围的培养基中后，检测微载体珠上生长的牛肺动脉内皮细胞对亚甲蓝（MB）和甲苯胺蓝O（TBO）的摄取，作为培养基中染料浓度的降低。由于这些染料的还原形式比氧化形式更亲脂，我们认为细胞表面染料的还原可能先于细胞的摄取。因此，我们研究了细胞还原甲苯胺蓝O-聚丙烯酰胺聚合物（TBOP）的能力，该聚合物太大而不能以氧化或还原形式进入细胞。聚合物的TBO部分被细胞还原，表明染料不必进入细胞以被还原，并且还原可以发生在细胞表面处或附近。TBOP减少的速率与单体染料的摄取速率大致相同，表明细胞表面还原机制具有足够的能力来解释细胞对单体的摄取。我们还发现，铁氰化物离子，也没有渗透到细胞，减少了细胞和外部铁氰化物抑制单体MB的摄取。因此，铁氰化物的结果也与以下概念一致，即单体噻嗪染料在内皮细胞吸收更亲脂的还原形式之前在细胞表面被还原。
The uptake of methylene blue (MB), and toluidine blue O (TBO) by bovine pulmonary arterial endothelial cells grown on microcarrier beads was detected as a decrease in the concentration of dye in the medium after these thiazine dyes were added to the medium surrounding the cells. Because the reduced forms of these dyes are much more lipophilic than the oxidized forms, we considered the possibility that reduction of the dyes at the cell surface might have preceded the uptake by the cells. Therefore, we studied the ability of the cells to reduce a toluidine blue O-polyacrylamide polymer (TBOP), which was too large to enter the cells in either the oxidized or reduced form. The TBO moieties of the polymer were reduced by the cells, indicating that the dyes did not have to enter the cells to be reduced and that reduction can occur at, or near, the cell surface. The rate of TBOP reduction was about the same as the rate of uptake of the monomeric dyes, indicating that the cell surface reduction mechanism had a suffcient capacity to account for the monomer uptake by the cells. We also found that ferricyanide ion, which also did not permeate the cells, was reduced by the cells and that external ferricyanide inhibited the monomeric MB uptake. Thus the results with ferricyanide were also consistent with the concept that the monomeric thiazine dyes are reduced at the cell surface before the more lipophilic reduced forms are taken up by the endothelial cells.