Development of a stable dual functional coating with low non-specific protein adsorption and high sensitivity for new superparamagnetic nanospheres.
Development of a stable dual functional coating with low non-specific protein adsorption and high sensitivity for new superparamagnetic nanospheres.
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DOI：
10.1021/la202566d
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发表时间：
2011-10
期刊：
影响因子：
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通讯作者：
Xian’an Zhang;Weifeng Lin;Shengfu Chen;Hong Xu;H. Gu
中科院分区：
文献类型：
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作者：
Xian’an Zhang;Weifeng Lin;Shengfu Chen;Hong Xu;H. Gu
为了克服纳米传感和纳米诊断纳米颗粒上非特异性蛋白质吸附的主要挑战，开发了一种用于稳健化学修饰的有效方法，以在复杂生物介质中实现优异的特异性生物识别和长期稳定性。该方法通过使用具有高磁铁矿含量的超顺磁性纳米球（NS）的高度特异性和灵敏度的免疫测定（IA）来证明。通过表面引发原子转移自由基聚合（SI-ATRP）修饰双功能聚（羧基甜菜碱甲基丙烯酸酯）（polyCBMA），并与人绒毛膜促性腺激素β亚基抗体（anti-β-hCG）化学接枝，可显著抑制NS对蛋白质的非特异性吸附。具有聚CBMA涂层的IA NS对hCG的响应在磷酸盐缓冲盐水（PBS）或50%胎牛血清（FBS）中高度一致，这远小于不具有聚CBMA涂层的IA NS的响应的变化。毕竟，获得了具有优异特异性生物识别的IA NS的非常稳健的平台。这种纳米粒子修饰方法有望在未来的超灵敏纳米传感和纳米诊断中得到广泛应用。
To overcome major challenges of non-specific protein adsorption on nanoparticles for nanosensing and nanodiagnosis, an efficient method for robust chemical modification was developed to achieve excellent specific biorecognition and long-term stability in complex biomedia. This method is demonstrated by a highly specific and sensitive immunoassay (IA), using superparamagnetic nanospheres (NSs) with high magnetite content. The non-specific protein adsorption on the NSs was suppressed dramatically when modified with dual functional poly(carboxybetaine methacrylate) (polyCBMA) via surface-initiated atom transfer radical polymerization (SI-ATRP) and chemically grafted with antibodies of the β subunit of human chorionic gonadotrop (anti-β-hCG). The response to hCG of IA NSs with polyCBMA coatings was highly consistent in either phosphate-buffered saline (PBS) or 50% fetal bovine serum (FBS), which is far less variable than the response of the IA NSs without polyCBMA coatings. After all, a very robust platform for IA NSs with excellent specific biorecognition was obtained. It is expected that this method for nanoparticle modification could be widely used in ultrasensitive nanosensing and nanodiagnosis in the future.