The effect of varying levels of sodium bicarbonate on polychlorinated biphenyl dechlorination in Hudson River sediment cultures.
The effect of varying levels of sodium bicarbonate on polychlorinated biphenyl dechlorination in Hudson River sediment cultures.
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DOI：
10.1111/j.1462-2920.2006.01037.x
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发表时间：
2006-07
影响因子：
5.1
通讯作者：
T. Yan;T. LaPara;P. Novak
中科院分区：
文献类型：
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作者：
T. Yan;T. LaPara;P. Novak
添加不同浓度的碳酸氢钠对哈德逊河沉积物培养物中2，3，4，5-氯联苯（2，3，4，5-CB）的脱氯产生了深远的影响。最广泛的脱氯观察到的文化，其中100毫克l（-1）碳酸氢盐。用1000 mg l（-1）碳酸氢盐修正的培养物具有最小程度的脱氯作用，2，4-CB和2，5-CB是主要的最终产物。在添加了<或= 500 mg l（-1）碳酸氢盐的培养物中观察到氯化联苯总质量的显著损失，表明发生了氯联苯以外的降解。乙酸形成的动力学是不同的治疗，在整个303天的实验中检测到高浓度的乙酸盐，其中1000毫克升（-1）碳酸氢盐的文化。碳酸氢钠的添加也有显着的影响，细菌群落结构的16 S rRNA基因片段的聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）检测。三个假定的多氯联苯（PCB）脱氯剂进行了鉴定，一个Dehalococcoides样人口中检测到的所有富集文化，而两个Dehalobacter-like人口中检测到的富集文化与最广泛的脱氯。这些结果表明，碳酸氢盐的可用性，并可能钠，可能会影响多氯联苯脱氯在哈德逊河沉积物，因此需要考虑到评估的命运时，多氯联苯或实施生物修复。
The addition of different concentrations of sodium bicarbonate had a profound effect on 2,3,4,5-chlorobiphenyl (2,3,4,5-CB) dechlorination in Hudson River sediment cultures. The most extensive dechlorination was observed in cultures to which 100 mg l(-1) bicarbonate was added. Cultures amended with 1000 mg l(-1) bicarbonate had the least extensive dechlorination, with 2,4-CB and 2,5-CB as predominant end-products. A significant loss of total chlorinated biphenyl mass was observed in cultures to which < or = 500 mg l(-1) bicarbonate was added, suggesting that degradation beyond chlorinated biphenyls occurred. The dynamics of acetate formation were different among the treatments, with high acetate concentrations detected throughout the 303-day experiment in cultures to which 1000 mg l(-1) bicarbonate had been added. Sodium bicarbonate addition also had a significant impact on bacterial community structure as detected by polymerase chain reaction-denaturant gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene fragments. Three putative polychlorinated biphenyl (PCB) dechlorinators were identified; one Dehalococcoides-like population was detected in all enrichment cultures, whereas two Dehalobacter-like populations were only detected in the enrichment cultures with the most extensive dechlorination. These results suggest that the availability of bicarbonate, and potentially sodium, may affect PCB dechlorination in Hudson River sediment and thus need to be taken into consideration when assessing the fate of PCBs or implementing bioremediation.