Regulation of human cytotoxic T lymphocyte development by IL-7.
Regulation of human cytotoxic T lymphocyte development by IL-7.
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IL-7 对人细胞毒性 T 淋巴细胞发育的调节。
DOI：
10.4049/jimmunol.145.8.2415
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发表时间：
1990
影响因子：
4.4
通讯作者：
J. Siegel
中科院分区：
文献类型：
--
作者：
C. J. Hickman;J. A. Crim;H. Mostowski;J. Siegel
在同种异体抗原、病毒和凝集素刺激的系统中检查了 IL-7 对人 CTL 生成的影响。在培养开始时添加 IL-7 导致细胞毒性显着增强（高达 80 倍），同时淋巴细胞总数略有增加（1.5 至 4 倍）。对纯化的凝集素刺激的 CD8+ T 细胞群中 CTL 发育的研究表明，IL-7 可以直接作用于 CD8+ 淋巴细胞亚群以增强细胞毒性。在 MLC 中，发现 IL-7 诱导的细胞毒性增强主要是由淋巴细胞 CD8+ 亚群介导的。后期添加 IL-7（7 天的第 5 天）导致细胞溶解活性增加，这与总或活化 CD8+ 淋巴细胞数量很少或没有增加相关，表明 IL-7 可能充当人 CTL 的分化因子。在开始时（或之后 5 天）向 MLC 中添加 IL-7 中和抗血清导致细胞毒性水平降低，这一观察表明内源性 IL-7 在 CTL 发育中的作用。这些结果表明 IL-7 可以对人类 CTL 发育发挥主要的上调作用，并表明这些作用既具有增殖性又具有分化性。
The effects of IL-7 on the generation of human CTL in alloantigen-, virus-, and lectin-stimulated systems were examined. Addition of IL-7 at the onset of cultures resulted in marked (up to 80-fold) augmentation of cytotoxicity accompanied by smaller (1.5- to 4-fold) increases in total lymphocyte number. Studies of CTL development in purified lectin-stimulated CD8+ T cell populations demonstrated that IL-7 could act directly on the CD8+ lymphocyte subset to augment cytotoxicity. In MLC, the IL-7-induced enhancement of cytotoxicity was found to be mediated primarily by the CD8+ subpopulation of lymphocytes. Late addition of IL-7 (day 5 of 7) resulted in an increase in cytolytic activity that was associated with little or no increase in total or activated CD8+ lymphocyte number indicating that IL-7 may act as a differentiation factor for human CTL. A role for endogenous IL-7 in CTL development was suggested by the observation that addition of neutralizing antiserum to IL-7 to MLC at initiation (or 5 days thereafter) resulted in decreased levels of cytotoxicity. These results indicate that IL-7 can exert major up-regulatory effects on human CTL development and suggest that these effects are both proliferative and differentiative.