INVITRO PACKAGING AND REPLICATION OF INDIVIDUAL GENOMIC SEGMENTS OF BACTERIOPHAGE-PHI-6 RNA
INVITRO PACKAGING AND REPLICATION OF INDIVIDUAL GENOMIC SEGMENTS OF BACTERIOPHAGE-PHI-6 RNA
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DOI：
10.1128/jvi.66.5.2611-2616.1992
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发表时间：
1992-05-01
影响因子：
5.4
通讯作者：
MINDICH, L
中科院分区：
文献类型：
--
作者：
GOTTLIEB, P;STRASSMAN, J;MINDICH, L
噬菌体phi-6的基因组含有三个双链RNA片段。由大基因组片段的cDNA拷贝指导形成的前衣壳结构能够在ATP存在下包装三种病毒信使正义RNA。UTP、CTP和GTP的加入导致负链的合成以形成双链RNA。在这份报告中，我们表明，原衣壳能够采取任何三个正链单链RNA片段独立于其他。在含锰缓冲液中，相应负链的合成发生。在含镁缓冲液中，包装了单个的正义病毒RNA片段，但除非包装了所有三个病毒单链RNA片段，否则不会发生负链复制。由于镁缓冲液中的包装条件更接近于体内包装条件，因此这些结果表明包装中没有特定的顺序或依赖性，并且复制受到调节，因此直到所有片段就位才开始。
The genome of bacteriophage phi-6 contains three segments of double-stranded RNA. Procapsid structures whose formation was directed by cDNA copies of the large genomic segment are capable of packaging the three viral message sense RNAs in the presence of ATP. Addition of UTP, CTP, and GTP results in the synthesis of minus strands to form double-stranded RNA. In this report, we show that procapsids are capable of taking up any of the three plus-strand single-stranded RNA segments independently of the others. In manganese-containing buffers, synthesis of the corresponding minus strand takes place. In magnesium-containing buffers, individual message sense viral RNA segments were packaged, but minus-strand replication did not take place unless all three viral single-stranded RNA segments were packaged. Since the conditions of packaging in magnesium buffer more closely resemble those in vivo, these results indicated that there is no specific order or dependence in packaging and that replication is regulated so that it does not begin until all segments are in place.