An antioxidant and antibacterial polydopamine-modified thermo-sensitive hydrogel dressing for Staphylococcus aureus-infected wound healing
An antioxidant and antibacterial polydopamine-modified thermo-sensitive hydrogel dressing for Staphylococcus aureus-infected wound healing
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DOI：
10.1039/d2nr04908b
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发表时间：
2022-12-06
期刊：
影响因子：
6.7
通讯作者：
He, Bin
中科院分区：
文献类型：
--
作者：
Ge, Pengjin;Chang, Shuhua;He, Bin
细菌感染的伤口愈合是一个复杂而慢性的过程，对人类健康构成巨大威胁。在体温下经历溶胶-凝胶转变的热敏水凝胶是一种有吸引力的伤口敷料，可加速愈合和预防感染。在本文中，我们提出了一种基于聚多巴胺改性的聚（ε-己内酯-共-乙交酯）-b-聚（乙二醇）-b-聚（ε-己内酯-共-乙交酯）（PDA/P2）三嵌段共聚物的热敏和活性氧（ROS）清除水凝胶。浓度为 30 wt% 的 PDA/P2 溶液可在 34-38°C 形成凝胶。通过 DPPH 和 ABTS 测定以及 RAW264.7 细胞中细胞内 ROS 下调证明了 PDA/P2 的 ROS 清除能力。此外，银纳米粒子被封装在水凝胶（PDA/P2-4@Ag 凝胶）中，以提供针对大肠杆菌和金黄色葡萄球菌的抗菌活性。体内金黄色葡萄球菌感染的大鼠模型表明，PDA/P2-4@Ag水凝胶敷料可以通过抑制细菌生长、减轻炎症反应、诱导血管生成和胶原沉积来促进伤口愈合。该研究为制备基于温度敏感的水凝胶的多功能伤口敷料提供了一种新策略。
Bacteria-infected wound healing is a complex and chronic process that poses a great threat to human health. A thermo-sensitive hydrogel that undergoes a sol-gel transition at body temperature is an attractive wound dressing for healing acceleration and infection prevention. In this paper, we present a thermo-sensitive and reactive oxygen species (ROS)-scavenging hydrogel based on polydopamine modified poly(epsilon-caprolactone-co-glycolide)-b-poly(ethylene glycol)-b-poly(epsilon-caprolactone-co-glycolide) (PDA/P2) triblock copolymer. The PDA/P2 solution at a concentration of 30 wt% could form a gel at 34-38 degrees C. The ROS-scavenging ability of PDA/P2 was demonstrated by DPPH and ABTS assays and intracellular ROS downregulation in RAW264.7 cells. Furthermore, silver nanoparticles were encapsulated in the hydrogel (PDA/P2-4@Ag gel) to provide antibacterial activity against E. coli and S. aureus. An in vivo S. aureus-infected rat model demonstrated that the PDA/P2-4@Ag hydrogel dressing could promote wound healing via inhibiting bacterial growth, alleviating the inflammatory response, and inducing angiogenesis and collagen deposition. This study provides a new strategy to prepare temperature-sensitive hydrogel-based multifunctional wound dressings.