Frequency of serological non-responders and false-negative RT-PCR results in SARS-CoV-2 testing: a population-based study
Frequency of serological non-responders and false-negative RT-PCR results in SARS-CoV-2 testing: a population-based study
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DOI：
10.1515/cclm-2020-0978
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发表时间：
2020-12-01
影响因子：
6.8
通讯作者：
Risch, Martin
中科院分区：
文献类型：
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作者：
Baron, Rita Christiane;Risch, Lorenz;Risch, Martin
目的：在流行病学背景下，分子和血清学方法用于COVID-19检测的灵敏度尚未得到很好的描述。本研究的目的是确定首次临床表现时阴性RT-PCR结果的频率以及至少3周随访后阴性血清学结果的频率。方法：在列支敦士登所有疑似COVID-19患者中（n=1921），我们纳入了最初RT-PCR阳性的索引患者（n=85）以及最初RT-PCR阴性的患者（n=66），以进行SARS-CoV-2抗体检测的随访。用七种不同的市售免疫测定法检测抗体。结果：在列支敦士登的COVID-19患者中，18%（12/66）的患者在初次就诊时出现假阴性RT-PCR，而COVID-19患者的血清学阴性率为4%（3/85）。验证队列显示出相似的频率：血清学阴性2/66（3%），假阴性RT-PCR 16/155（10%）。与其他COVID-19患者相比，随访血清学阴性的COVID-19患者的病程更长（p=0.05），临床症状更多（p
Objectives: The sensitivity of molecular and serological methods for COVID-19 testing in an epidemiological setting is not well described. The aim of the study was to determine the frequency of negative RT-PCR results at first clinical presentation as well as negative serological results after a follow-up of at least 3 weeks.Methods: Among all patients seen for suspected COVID-19 in Liechtenstein (n=1921), we included initially RT-PCR positive index patients (n=85) as well as initially RT-PCR negative (n=66) for follow-up with SARS-CoV-2 antibody testing. Antibodies were detected with seven different commercially available immunoassays. Frequencies of negative RT-PCR and serology results in individuals with COVID-19 were determined and compared to those observed in a validation cohort of Swiss patients (n=211).Results: Among COVID-19 patients in Liechtenstein, false-negative RT-PCR at initial presentation was seen in 18% (12/66), whereas negative serology in COVID-19 patients was 4% (3/85). The validation cohort showed similar frequencies: 2/66 (3%) for negative serology, and 16/155 (10%) for false negative RT-PCR. COVID-19 patients with negative follow-up serology tended to have a longer disease duration (p=0.05) and more clinical symptoms than other patients with COVID-19 (p