Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering.
Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering.
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DOI：
10.1186/s11671-016-1532-4
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发表时间：
2016-12
影响因子：
--
通讯作者：
Kang IK
中科院分区：
文献类型：
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作者：
Kwak S;Haider A;Gupta KC;Kim S;Kang IK
采用双挤出静电纺丝技术，将聚乳酸-羟基乙酸共聚物（PLGA）的微纤维网与PLGA和胶原的微/纳米混合纤维网交替堆叠，制备多层三维支架。为了制造多层支架，使用含和不含羟基磷灰石纳米棒（nHA）的35重量%PLGA在THF-DMF二元溶剂（3：1）中的溶液和5重量%胶原在六氟异丙醇（HFIP）中的溶液。PLGA和胶原蛋白的双重和单独电纺丝分别以1.0和0.5 mL/h的流速在20 kV的施加电压下进行。多层支架中胶原纤维的密度控制了MC 3 T3-E1细胞的粘附、增殖和成骨分化。谷氨酸修饰的羟基磷灰石纳米棒（nHA-GA）在胶原溶液中的均匀分散提高了所制造的多层支架的成骨性能。所制造的多层支架，其特征在于使用FT-IR，X射线光电子能谱，和透射电子显微镜（TEM）。采用扫描电子显微镜（FE-SEM）观察MC 3 T3-E1细胞在多层支架上的粘附和铺展情况。应用MTT、碱性磷酸酶、茜素红、von Kossa和细胞骨架F-actin测定方案评价MC 3 T3-E1细胞在多层支架上的活性。与原始微纤维PLGA和微/纳米混合纤维PLGA和Col支架相比，发现微/纳米纤维PLGA-Col-HA支架具有高度生物活性。
The dual extrusion electrospinning technique was used to fabricate multilayered 3D scaffolds by stacking microfibrous meshes of poly(lactic acid-co-glycolic acid) (PLGA) in alternate fashion to micro/nano mixed fibrous meshes of PLGA and collagen. To fabricate the multilayered scaffold, 35 wt% solution of PLGA in THF-DMF binary solvent (3:1) and 5 wt% solution of collagen in hexafluoroisopropanol (HFIP) with and without hydroxyapatite nanorods (nHA) were used. The dual and individual electrospinning of PLGA and collagen were carried out at flow rates of 1.0 and 0.5 mL/h, respectively, at an applied voltage of 20 kV. The density of collagen fibers in multilayered scaffolds has controlled the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells. The homogeneous dispersion of glutamic acid-modified hydroxyapatite nanorods (nHA-GA) in collagen solution has improved the osteogenic properties of fabricated multilayered scaffolds. The fabricated multilayered scaffolds were characterized using FT-IR, X-ray photoelectron spectroscopy, and transmission electron microscopy (TEM). The scanning electron microscopy (FE-SEM) was used to evaluate the adhesion and spreads of MC3T3-E1 cells on multilayered scaffolds. The activity of MC3T3-E1 cells on the multilayered scaffolds was evaluated by applying MTT, alkaline phosphatase, Alizarin Red, von Kossa, and cytoskeleton F-actin assaying protocols. The micro/nano fibrous PLGA-Col-HA scaffolds were found to be highly bioactive in comparison to pristine microfibrous PLGA and micro/nano mixed fibrous PLGA and Col scaffolds.