GAMMA-RAY-INDUCED LOSS OF EXPRESSION OF HLA AND GLYOXALASE-I ALLELES IN LYMPHOBLASTOID-CELLS
GAMMA-RAY-INDUCED LOSS OF EXPRESSION OF HLA AND GLYOXALASE-I ALLELES IN LYMPHOBLASTOID-CELLS
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DOI：
10.1073/pnas.77.7.4251
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
DEMARS, R
中科院分区：
文献类型：
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作者：
KAVATHAS, P;BACH, FH;DEMARS, R
来自 Ce 源的伽马射线被用来产生人类淋巴母细胞系变体，这些变体失去了由单一单倍型编码的所有主要组织相容性复合体抗原的表达。该细胞系在乙二醛酶 I 基因座上是杂合的，并且具有 HLA 单倍体 HLA-A1、B8、DRw3 和 HLA-A2、B5 DRw1。抗HLA-B8抗血清用于在已用300 Rγ-射线照射的细胞群中进行选择。 B8缺失变异的发生率为4.1倍。照射后第5天10-5。变体分析表明，B8 反式的 HLA 和 GLO 等位基因的表达得以保留。 17 个变体中的 12 个变体中额外的顺式连接 HLA 和 GLO 基因产物的表达丢失。失去 HLA-B8 表达的变体； HLA-B8、A1； HLA-B8、A1、DRw3；或获得HLA-B8、A1、DRw3和顺式连接的乙二醛酶I等位基因。对失去 2 个或更多顺联等位基因表达的 8 个变体进行核型分析。 3 个变体有 2 条外观正常的第 6 号染色体，4 个变体有缺失，其中包括一条 6 号染色体短臂上编码 HLA 基因的区域，1 个变体有涉及 6 号染色体短臂的倒位或易位。
Gamma rays from a Ce source were used to generate human lymphoblastoid cell line variants that had lost expression of all major histocompatibility complex antigens coded for by a single haplotype. The cell line was heterozygous at the glyoxalase I locus and had the HLA haplotyes HLA-A1, B8, DRw3 and HLA-A2, B5 DRw1. Anti-HLA-B8 antiserum was used for selection in a population of cells that had been irradiated with 300 R .gamma.-rays. The incidence of B8-loss variants was 4.1 .times. 10-5 on day 5 after irradiation. Analysis of variants showed that expressions of HLA and GLO alleles trans to B8 were retained. Expression of additional cis-linked HLA and GLO gene products was lost in 12 of 17 variants. Variants that had lost expression of HLA-B8; HLA-B8, A1; HLA-B8, A1, DRw3; or HLA-B8, A1, DRw3 and the cis-linked glyoxalase I allele were obtained. Karyotype analysis was preformed on 8 variants that had lost expression of 2 or more cis-linked alleles. Three variants had 2 normal appearing 6th chromosomes, 4 variants had a deletion that included the region coding for HLA genes on the short arm of one chromosome 6 and 1 variant had an inversion or translocation involving the short arm of one chromosome 6.