Distribution of BK Polyomavirus Genotypes in Tunisian Renal Transplant Recipients Between January 2008 and May 2009
Distribution of BK Polyomavirus Genotypes in Tunisian Renal Transplant Recipients Between January 2008 and May 2009
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DOI：
10.1002/jmv.22035
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发表时间：
2011-04-01
影响因子：
12.7
通讯作者：
Aouni, Mahjoub
中科院分区：
文献类型：
--
作者：
Boukoum, Hanen;Nahdi, Imen;Aouni, Mahjoub
BK多瘤病毒（BKV）是一种在人类中普遍存在的病毒，在儿童期原发感染后仍潜伏在泌尿生殖道中。这种病毒经常被重新激活并通过尿液排出，可导致肾移植受者的肾病。BKV序列分为四种亚型（I-IV）。亚型I和IV分别进一步分为四个和六个亚组。为了表征突尼斯流行的BKV亚型，通过实时PCR研究了77名肾移植受者尿液样本中病毒的存在。为了进行亚型鉴定，采用巢式PCR从阳性样品中扩增VP 1编码区的DNA片段，进行测序并进行系统发育分析。在研究人群中，确定了I型（75.5%）、II型（14.5%）和IV型（2.5%），与欧洲人群中观察到的Ib-2型（73%）明显占优势。这项研究表明，在北非，BKV基因型分布与欧洲相似，与撒哈拉以南非洲不同。J. Med. Virol. 83：725-730，2011. (C)2011 Wiley-Liss，Inc.
BK polyomavirus (BKV) is a ubiquitous virus in humans that remains latent in the urogenital tract after a primary infection during childhood. The virus, which is reactivated frequently and excreted in urine, can cause nephropathy in renal transplant recipients. BKV sequences are classified into four subtypes (I-IV). Subtype I and IV are divided further into four and six subgroups, respectively. To characterize the subtypes of BKV prevalent in Tunisia, the presence of the virus was investigated by real-time PCR in urine samples from 77 renal transplant recipients. For subtype identification, a DNA fragment in the VP1 coding region, amplified by nested PCR from positive samples, was sequenced and a phylogenetic analysis was performed. In the studied population, subtype I (75.5%), II (14.5%), and IV (2.5%) were identified with a clear predominance of subtype Ib-2 (73%) as observed in European population. This study suggests that in North Africa, the BKV genotype distribution is similar to that of Europe and different from that of sub-Saharan Africa. J. Med. Virol. 83:725-730, 2011. (C) 2011 Wiley-Liss, Inc.