Detection of Ferroptosis by BODIPY™ 581/591 C11
Detection of Ferroptosis by BODIPY™ 581/591 C11
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DOI：
10.1007/978-1-0716-0247-8_11
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Yang, Wan Seok
中科院分区：
文献类型：
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作者：
Martinez, Alejandra M.;Kim, Ahryun;Yang, Wan Seok
铁凋亡是一种独特的调节性细胞死亡形式，其由质膜中脂质过氧化物的致死性积累驱动。未能控制铁凋亡已经涉及多种病理学状况，包括癌症发展、神经变性、肾损伤、缺血/再灌注损伤和T细胞免疫。在这里，我们描述了一种方法来检测铁凋亡，通过使用BODIPY-C11探针和流式细胞仪测定细胞膜中的脂质过氧化物的量。可以使用相同的方法确定铁凋亡在免疫调节细胞中的假定作用。
Ferroptosis is a distinctive form of regulated cell death that is driven by lethal accumulation of lipid peroxides in plasma membranes. Failure to control ferroptosis has been implicated in multiple pathological conditions including cancer development, neurodegeneration, renal injury, ischemia/reperfusion injury, and T-cell immunity. Here we describe a method to detect ferroptosis by determining the amount of lipid peroxides in cellular membranes using BODIPY-C11 probe and flow cytometry. Putative role of ferroptosis in immune modulatory cells can be determined using the same method.