Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO₂.
Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO₂.
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DOI：
10.1038/srep08132
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发表时间：
2015-01-30
影响因子：
4.6
通讯作者：
Pakrasi HB
中科院分区：
文献类型：
--
作者：
Yu J;Liberton M;Cliften PF;Head RD;Jacobs JM;Smith RD;Koppenaal DW;Brand JJ;Pakrasi HB
光合微生物作为生物技术中的生产生物而引起人们的兴趣，因为它们可以利用太阳光（一种丰富的能源）和CO2（一种温室气体）进行自养生长。这种微生物的重要特征是快速生长和对遗传操作的顺从性。在这里，我们描述了细长聚球藻UTEX 2973，单细胞蓝藻能够快速自养生长，异养工业主机，如酵母。聚球藻UTEX 2973可以容易地转化以容易地产生所需的敲除和敲入突变。基因组测序结合全球蛋白质组学研究表明，Synechococcus UTEX 2973是广泛研究的蓝细菌Synechococcus elongatus PCC 7942的近亲，后者生长速度慢两倍以上。少量的核苷酸变化是这两种蓝藻菌株基因组之间唯一的显著差异。因此，我们的研究揭示了具有重要工业潜力的蓝藻菌株快速生长所必需的遗传决定因素。
Photosynthetic microbes are of emerging interest as production organisms in biotechnology because they can grow autotrophically using sunlight, an abundant energy source, and CO2, a greenhouse gas. Important traits for such microbes are fast growth and amenability to genetic manipulation. Here we describe Synechococcus elongatus UTEX 2973, a unicellular cyanobacterium capable of rapid autotrophic growth, comparable to heterotrophic industrial hosts such as yeast. Synechococcus UTEX 2973 can be readily transformed for facile generation of desired knockout and knock-in mutations. Genome sequencing coupled with global proteomics studies revealed that Synechococcus UTEX 2973 is a close relative of the widely studied cyanobacterium Synechococcus elongatus PCC 7942, an organism that grows more than two times slower. A small number of nucleotide changes are the only significant differences between the genomes of these two cyanobacterial strains. Thus, our study has unraveled genetic determinants necessary for rapid growth of cyanobacterial strains of significant industrial potential.